Cao Yu, Zhang Jiang, Yang Wei, Xia Cheng, Zhang Hong-You, Wang Yan-Hui, Xu Chuang
Department of College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
J Vet Res. 2017 Apr 4;61(1):91-95. doi: 10.1515/jvetres-2017-0011. eCollection 2017 Mar.
The predictive value of selected parameters in the risk of ketosis and fatty liver in dairy cows was determined.
In total, 21 control and 17 ketotic Holstein Friesian cows with a β-hydroxybutyrate (BHBA) concentration of 1.20 mmol/L as a cut-off point were selected. The risk prediction thresholds for ketosis were determined by receiver operating characteristic (ROC) curve analysis.
In the ketosis group, paraoxonase-1 (PON-1) activity and concentration of PON-1 and glucose (GLU) were decreased, and aminotransferase (AST) activity as well as BHBA and non-esterified fatty acid (NEFA) contents were increased. The plasma activity and concentration of PON-1 were significantly positively correlated with the level of plasma GLU. The plasma activity and concentration of PON-1 were significantly negatively correlated with the levels of AST and BHBA. According to ROC curve analysis, warning indexes of ketosis were: plasma PON-1 concentration of 46.79 nmol/L, GLU concentration of 3.04 mmol/L, AST concentration of 100 U/L, and NEFA concentration of 0.82 mmol/L.
This study showed that the levels of PON-1, GLU, AST, and NEFA could be used as indicators to predict the risk of ketosis in dairy cows.
确定了所选参数对奶牛酮病和脂肪肝风险的预测价值。
总共选取了21头对照奶牛和17头酮病荷斯坦弗里生奶牛，以β-羟基丁酸（BHBA）浓度1.20 mmol/L作为分界点。通过受试者工作特征（ROC）曲线分析确定酮病的风险预测阈值。
在酮病组中，对氧磷酶-1（PON-1）活性、PON-1和葡萄糖（GLU）浓度降低，而转氨酶（AST）活性以及BHBA和非酯化脂肪酸（NEFA）含量增加。血浆PON-1活性和浓度与血浆GLU水平显著正相关。血浆PON-1活性和浓度与AST和BHBA水平显著负相关。根据ROC曲线分析，酮病的预警指标为：血浆PON-1浓度46.79 nmol/L、GLU浓度3.04 mmol/L、AST浓度100 U/L和NEFA浓度0.82 mmol/L。
本研究表明，PON-1、GLU、AST和NEFA水平可作为预测奶牛酮病风险的指标。
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