Dziekońska Anna, Kinder Marek, Fraser Leyland, Strzeżek Jerzy, Kordan Władysław
Department of Animal Biochemistry and BiotechnologyUniversity of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland.
J Vet Res. 2017 Apr 4;61(1):127-133. doi: 10.1515/jvetres-2017-0016. eCollection 2017 Mar.
The aim of this study was to evaluate the effect of lipoprotein fraction isolated from ostrich egg yolk (LPFo) on the metabolic activity of boar spermatozoa following liquid semen storage in different extenders and temperatures.
Boar ejaculates were extended in Androhep, Beltsville thawing solution (BTS), and Martín-Rillo and Alias (MR-A) without (control) or with the addition of LPFo and stored for three days at either 5°C or 16°C. The analysed sperm parameters included total motility (TMOT), plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), oxygen consumption, and adenosine triphosphate (ATP) production.
The sperm metabolic activity seemed to be higher in the LPFo-based extenders following storage for three days, irrespective of the storage temperature. Compared with the LPFo-free extenders, significantly higher (P < 0.05) sperm PMI and MMP were observed in BTS and MR-A extenders supplemented with LPFo during storage for three days at 5°C. Spermatozoa stored in the BTS-LPFo extender exhibited higher (P < 0.05) TMOT and oxygen consumption, whereas higher (P < 0.05) PMI was observed in spermatozoa stored in Androhep-LPFo and MR-A-LPFo for three days at 16°C. No significant differences (P > 0.05) in ATP content were observed between the LPFo-free and LPFo-based extenders during storage.
Supplementation of LPFo to semen extenders had varying effects on the metabolic activity of boar spermatozoa stored at different temperatures. It can be suggested that the interactions of various components of the extenders and seminal plasma with LPFo exert beneficial effects on the sperm metabolic activity during liquid storage of boar semen.
本研究的目的是评估从鸵鸟蛋黄中分离出的脂蛋白组分（LPFo）对公猪精液在不同稀释液和温度下液态保存后精子代谢活性的影响。
将公猪精液用Androhep、贝尔茨维尔解冻液（BTS）以及Martín-Rillo和Alias（MR-A）进行稀释，不添加（对照）或添加LPFo，并在5°C或16°C下保存三天。分析的精子参数包括总活力（TMOT）、质膜完整性（PMI）、线粒体膜电位（MMP）、耗氧量和三磷酸腺苷（ATP）生成量。
无论保存温度如何，在基于LPFo的稀释液中保存三天后，精子代谢活性似乎更高。与不含LPFo的稀释液相比，在5°C下保存三天时，添加LPFo的BTS和MR-A稀释液中观察到精子PMI和MMP显著更高（P < 0.05）。保存在BTS-LPFo稀释液中的精子表现出更高（P < 0.05）的TMOT和耗氧量，而在16°C下保存三天的保存在Androhep-LPFo和MR-A-LPFo中的精子中观察到更高（P < 0.05）的PMI。在保存期间，不含LPFo和基于LPFo的稀释液之间在ATP含量上未观察到显著差异（P > 0.05）。
向精液稀释液中添加LPFo对在不同温度下保存的公猪精子代谢活性有不同影响。可以认为，稀释液和精浆的各种成分与LPFo的相互作用对公猪精液液态保存期间的精子代谢活性产生有益影响。
