González Wendy, Giménez-Lirola Luis G, Holmes Ashley, Lizano Sergio, Goodell Christa, Poonsuk Korakrit, Sitthicharoenchai Panchan, Sun Yaxuan, Zimmerman Jeffrey
College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
IDEXX Laboratories, Westbrook, ME, USA.
J Vet Res. 2017 Dec 6;61(2):163-171. doi: 10.1515/jvetres-2017-0021. eCollection 2017 Jun.
The prevention and control of in commercial production settings is based on serological monitoring. Enzyme-linked immunosorbent assays (ELISAs) have been developed to detect specific antibodies against a variety of antigens, including long-chain lipopolysaccharides (LPS) and the ApxIV toxin, a repeats-in-toxin (RTX) exotoxin unique to and produced by all serovars. The objective of this study was to describe ApxIV antibody responses in serum and oral fluid of pigs.
Four groups of pigs (six pigs per group) were inoculated with serovars 1, 5, 7, or 12. Weekly serum samples and daily oral fluid samples were collected from individual pigs for 56 days post inoculation (DPI) and tested by LPS and ApxIV ELISAs. The ApxIV ELISA was run in three formats to detect immunlgobulins M, G, and A (IgM, IgG and IgA) while the LPS ELISA detected only IgG.
All pigs inoculated with serovars 1 and 7 were LPS ELISA serum antibody positive from DPI 14 to 56. A transient and weak LPS ELISA antibody response was observed in pigs inoculated with serovar 5 and a single antibody positive pig was observed in serovar 12 at ≥35 DPI. Notably, ApxIV serum and oral fluid antibody responses in pig inoculated with serovars 1 and 7 reflected the patterns observed for LPS antibody, albeit with a 14 to 21 day delay.
This work suggests that ELISAs based on ApxIV antibody detection in oral fluid samples could be effective in population monitoring for .
商业生产环境中的防控工作基于血清学监测。已经开发出酶联免疫吸附测定（ELISA）来检测针对多种抗原的特异性抗体，包括长链脂多糖（LPS）和ApxIV毒素，ApxIV毒素是一种毒素重复序列（RTX）外毒素，是独有的且所有血清型都会产生。本研究的目的是描述猪血清和口腔液中ApxIV抗体反应。
四组猪（每组6头猪）接种血清型1、5、7或12。接种后56天（dpi）每周从每头猪采集血清样本，每天采集口腔液样本，并通过LPS和ApxIV ELISA进行检测。ApxIV ELISA以三种形式运行，以检测免疫球蛋白M、G和A（IgM、IgG和IgA），而LPS ELISA仅检测IgG。
所有接种血清型1和7的猪在dpi 14至56时LPS ELISA血清抗体呈阳性。接种血清型5的猪中观察到短暂且微弱的LPS ELISA抗体反应，在血清型12中，在≥35 dpi时观察到一头抗体阳性猪。值得注意的是，接种血清型1和7的猪中ApxIV血清和口腔液抗体反应反映了LPS抗体观察到的模式，尽管延迟了14至21天。
这项工作表明，基于口腔液样本中ApxIV抗体检测的ELISA在的群体监测中可能有效。
