Kittawornrat Apisit, Prickett John, Wang Chong, Olsen Chris, Irwin Christa, Panyasing Yaowalak, Ballagi Andrea, Rice Anna, Main Rodger, Johnson John, Rademacher Chris, Hoogland Marlin, Rowland Raymond, Zimmerman Jeffrey
Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011-1240, USA.
J Vet Diagn Invest. 2012 Mar;24(2):262-9. doi: 10.1177/1040638711435679.
The purpose of the present study was to evaluate the diagnostic performance of a commercial serum antibody enzyme-linked immunosorbent assay (ELISA) modified to detect anti-Porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in pen-based oral fluid specimens. Experimental and field oral fluid samples of defined status in reference to exposure of swine with PRRSV were used to derive the kinetics of detectable concentrations of antibody against PRRSV. Immunoglobulin (Ig)M and IgA were readily detected in oral fluid specimens from populations in which PRRSV infection was synchronized among all individuals but not in samples collected in commecial herds. In contrast, IgG was readily detected at diagnostically useful levels in both experimental and field samples for up to 126 days. Estimates of the IgG oral fluid ELISA performance were based on results from testing positive oral fluid samples (n = 492) from experimentally inoculated pigs (n = 251) and field samples (n = 241) and negative oral fluid samples (n = 367) from experimentally inoculated pigs (n = 84) and field samples (n = 283). Receiver operating characteristic analysis estimated the diagnostic sensitivity and specificity of the assay as 94.7% (95% confidence interval [CI]: 92.4, 96.5) and 100% (95% CI: 99.0, 100.0), respectively, at a sample-to-positive ratio cutoff of ≥0.40. The results of the study suggest that the IgG oral fluid ELISA can provide efficient, cost-effective PRRSV monitoring in commercial herds and PRRSV surveillance in elimination programs.
本研究的目的是评估一种经过改良的商用血清抗体酶联免疫吸附测定（ELISA）检测基于栏舍的口腔液体样本中抗猪繁殖与呼吸综合征病毒（PRRSV）抗体的诊断性能。利用参照猪只PRRSV暴露情况的具有明确状态的实验性和现场口腔液体样本，得出抗PRRSV抗体可检测浓度的动力学数据。在所有个体PRRSV感染同步的群体的口腔液体样本中，免疫球蛋白（Ig）M和IgA很容易被检测到，但在商业猪群采集的样本中未检测到。相比之下，在实验性和现场样本中，长达126天内都能很容易地检测到诊断有用水平的IgG。IgG口腔液体ELISA性能的估计基于对来自实验接种猪（n = 251）和现场样本（n = 241）的阳性口腔液体样本（n = 492）以及来自实验接种猪（n = 84）和现场样本（n = 283）的阴性口腔液体样本（n = 367）的检测结果。受试者工作特征分析估计，在样本与阳性比例截断值≥0.40时，该检测方法的诊断敏感性和特异性分别为94.7%（95%置信区间[CI]：92.4，96.5）和100%（95%CI：99.0，100.0）。研究结果表明，IgG口腔液体ELISA可为商业猪群提供高效、经济有效的PRRSV监测以及在根除计划中进行PRRSV监测。
