Liu Kun, Zhang Wei, Li Yunlong, Ding Qingfeng, Bai Yunan, Wang Fachen, Xu Guangming
Shandong Provincial Hospital Affiliated to Shandong University, No. 324 Jingwuweiqi Road, Jinan, 250021, Shandong Province, China.
Weifang Yidu Central Hospital, No. 4138 Linglongshan Road, Qingzhoushi, 262500, Shandong Province, China.
Cytotechnology. 2018 Oct;70(5):1291-1299. doi: 10.1007/s10616-017-0175-3. Epub 2018 Jul 5.
The potency of mesenchymal stem cells (MSCs) for tissue repair and regeneration is mainly based on their ability to secret beneficial molecules. Administration of MSCs has been proposed as an innovative approach and is proved by a number of clinical trials to a certain degree for the therapy of many diseases including Parkinson's disease (PD). However, the efficacy of MSCs alone is not significant. We investigated the effect of neurotrophic tyrosine receptor kinase 1 (NTRK1) overexpressed peripheral blood MSCs (PB-MSCs) on PD rat model. NTRK1 was overexpressed in PB-MSCs, which were then injected into PD rat model, Dopaminergic (DA) neuron regeneration and rotational performance was assessed. We found that DA neuron repair was increased in lesion site, rotational performance was also improved in MSC transplanted PD rat, with most potent effect in NTRK1 overexpressed PB-MSC transplanted PD rat. Our results indicate that overexpression of NTRK1 in MSCs could be an optimized therapeutic way via MSCs for PD treatment.
间充质干细胞（MSCs）用于组织修复和再生的潜能主要基于其分泌有益分子的能力。MSCs的给药已被提议作为一种创新方法，并且在一定程度上已被多项临床试验证明可用于治疗包括帕金森病（PD）在内的多种疾病。然而，单独使用MSCs的疗效并不显著。我们研究了过表达神经营养性酪氨酸受体激酶1（NTRK1）的外周血间充质干细胞（PB-MSCs）对PD大鼠模型的影响。NTRK1在PB-MSCs中过表达，然后将其注射到PD大鼠模型中，评估多巴胺能（DA）神经元再生和旋转行为。我们发现，在损伤部位DA神经元修复增加，在移植了MSCs的PD大鼠中旋转行为也得到改善，在移植了过表达NTRK1的PB-MSCs的PD大鼠中效果最为显著。我们的结果表明，在MSCs中过表达NTRK1可能是一种通过MSCs优化治疗PD的方法。
