Intraarticular and intravenous administration of Tc-HMPAO-labeled human mesenchymal stem cells (TC-AH-MSC): In vivo imaging and biodistribution.

INTRODUCTION

Therapeutic application of intravenous administered (IV) human bone marrow-derived mesenchymal stem cells (ahMSCs) appears to have as main drawback the massive retention of cells in the lung parenchyma, questioning the suitability of this via of administration. Intraarticular administration (IAR) could be considered as an alternative route for therapy in degenerative and traumatic joint lesions. Our work is outlined as a comparative study of biodistribution of Tc-ahMSCs after IV and IAR administration, via scintigraphic study in an animal model.

METHODS

Isolated primary culture of adult human mesenchymal stem cells was labeled with Tc-HMPAO for scintigraphic study of in vivo distribution after intravenous and intra-articular (knee) administration in rabbits.

RESULTS

IV administration of radiolabeled ahMSCs showed the bulk of radioactivity in the lung parenchyma while IAR images showed activity mainly in the injected cavity and complete absence of uptake in pulmonary bed.

CONCLUSIONS

Our study shows that IAR administration overcomes the limitations of IV injection, in particular, those related to cells destruction in the lung parenchyma. After IAR administration, cells remain within the joint cavity, as expected given its size and adhesion properties.

ADVANCES IN KNOWLEDGE

Intra-articular administration of adult human mesenchymal stem cells could be a suitable route for therapeutic effect in joint lesions.

IMPLICATIONS FOR PATIENT CARE

Local administration of adult human mesenchymal stem cells could improve their therapeutic effects, minimizing side effects in patients.