大鼠肾上腺髓质中的高亲和力血管紧张素受体。
High-affinity angiotensin receptors in rat adrenal medulla.
作者信息
Israel A, Niwa M, Plunkett L M, Saavedra J M
出版信息
Regul Pept. 1985 Jul;11(3):237-43. doi: 10.1016/0167-0115(85)90055-2.
Angiotensin II receptors have been quantitated in single rat adrenal medullas by incubation of tissue sections with 125I-[Sar1]-AII, autoradiography with exposure to 3H-sensitive Ultrofilm, computerized densitometry and comparison with 125I-labelled standards. Rat adrenal medulla contains a single class of high affinity AII receptors with a Ka of 0.84 +/- 0.02 X 10(9) M-1 and a Bmax of 3259 +/- 502 fmol/mg protein, one of the highest densities in AII receptors found in rat tissues. These observations provide evidence for a local site of action of AII in the release of adrenal medullary catecholamines.
通过用125I - [Sar1] - AII孵育组织切片、对3H敏感的超敏胶片曝光进行放射自显影、计算机化密度测定并与125I标记标准物比较，对单个大鼠肾上腺髓质中的血管紧张素II受体进行了定量。大鼠肾上腺髓质含有一类单一的高亲和力血管紧张素II受体，其解离常数（Ka）为0.84±0.02×10⁹ M⁻¹，最大结合容量（Bmax）为3259±502 fmol/mg蛋白质，这是在大鼠组织中发现的血管紧张素II受体最高密度之一。这些观察结果为血管紧张素II在肾上腺髓质儿茶酚胺释放中的局部作用位点提供了证据。
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