Mendelsohn F A, Quirion R, Saavedra J M, Aguilera G, Catt K J
Proc Natl Acad Sci U S A. 1984 Mar;81(5):1575-9. doi: 10.1073/pnas.81.5.1575.
The 125I-labeled agonist analog [1-sarcosine]-angiotensin II ( [Sar1]AII) bound with high specificity and affinity (Ka = 2 X 10(9) M-1) to a single class of receptor sites in rat brain. This ligand was used to analyze the distribution of AII receptors in rat brain by in vitro autoradiography followed by computerized densitometry and color coding. A very high density of AII receptors was found in the subfornical organ, paraventricular and periventricular nuclei of the hypothalamus, nucleus of the tractus solitarius, and area postrema. A high concentration of receptors was found in the suprachiasmatic nucleus of the hypothalamus, lateral olfactory tracts, nuclei of the accessory and lateral olfactory tracts, triangular septal nucleus, subthalamic nucleus, locus coeruleus, and inferior olivary nuclei. Moderate receptor concentrations were found in the organum vasculosum of the lamina terminalis, median preoptic nucleus, medial habenular nucleus, lateral septum, ventroposterior thalamic nucleus, median eminence, medial geniculate nucleus, superior colliculus, subiculum, pre- and parasubiculum, and spinal trigeminal tract. Low concentrations of sites were seen in caudate-putamen, nucleus accumbens, amygdala, and gray matter of the spinal cord. These studies have demonstrated that AII receptors are distributed in a highly characteristic anatomical pattern in the brain. The high concentrations of AII receptors at numerous physiologically relevant sites are consistent with the emerging evidence for multiple roles of AII as a neuropeptide in the central nervous system.
125I标记的激动剂类似物[1-肌氨酸]-血管紧张素II（[Sar1]AII）以高特异性和亲和力（Ka = 2×10⁹ M⁻¹）与大鼠脑中一类单一的受体位点结合。该配体用于通过体外放射自显影，随后进行计算机密度测定和颜色编码来分析大鼠脑中AII受体的分布。发现在穹窿下器官、下丘脑室旁核和室周核、孤束核和最后区中AII受体密度非常高。在下丘脑视交叉上核、外侧嗅束、副嗅束和外侧嗅束核、三角隔核、丘脑底核、蓝斑和下橄榄核中发现受体浓度高。在终板血管器、视前正中核、内侧缰核、外侧隔、丘脑腹后核、正中隆起、内侧膝状体、上丘、海马下脚、前海马下脚和副海马下脚以及脊髓三叉神经束中发现受体浓度中等。在尾状核-壳核、伏隔核、杏仁核和脊髓灰质中可见位点浓度低。这些研究表明，AII受体在脑中以高度特征性的解剖模式分布。在许多生理相关位点处高浓度的AII受体与AII作为中枢神经系统中一种神经肽的多种作用的新证据一致。
