He Xiao-yan, Chen Jian-li, Xiang Huan, Gao Yao, Tian Jun-sheng, Qin Xue-mei, Du Guan-hua
Yao Xue Xue Bao. 2017 Feb;52(2):245-52.
This study was designed to analyze the change of metabolites in the PC12 cells and its medium induced by corticosterone (CORT) and glutamate (Glu) by proton nuclear magnetic resonance ( 1H NMR) metabolomics. The multivariate statistical analysis was employed to identify the difference between control groups and induced groups, respectively. In addition, metabolite pathway analysis was performed to explore the characteristic of CORT-induced and or Glu-induced PC12 cells depression model, and to provide the references for the selection of in vitro depression models as well as the further understanding of the mechanism on depressive disorders. We found 36 differential metabolites in CORT-induced PC12 cells and medium and 42 in Glu-induced PC12 cells. Furthermore, correlation analysis results show that serine and 2-oxoisoleucine were associated with most differential metabolites in CORT-induced PC12 cells. Lactate and glutathione were significantly correlated to the vast majority of differential metabolites in Glu-induced PC12 cells. We speculated that CORT-induced PC12 cell models may affect the fatty acid metabolism and cell membrane structure, and Glu-induced PC12 cell models may have a difference in the glycolysis and antioxidants.
本研究旨在通过质子核磁共振（1H NMR）代谢组学分析皮质酮（CORT）和谷氨酸（Glu）诱导的PC12细胞及其培养基中代谢物的变化。采用多元统计分析分别鉴定对照组和诱导组之间的差异。此外，进行代谢物途径分析以探索CORT诱导和/或Glu诱导的PC12细胞抑郁模型的特征，并为体外抑郁模型的选择以及对抑郁症机制的进一步理解提供参考。我们在CORT诱导的PC12细胞和培养基中发现了36种差异代谢物，在Glu诱导的PC12细胞中发现了42种。此外，相关性分析结果表明，丝氨酸和2-氧代异亮氨酸与CORT诱导的PC12细胞中的大多数差异代谢物相关。乳酸和谷胱甘肽与Glu诱导的PC12细胞中的绝大多数差异代谢物显著相关。我们推测，CORT诱导的PC12细胞模型可能影响脂肪酸代谢和细胞膜结构，而Glu诱导的PC12细胞模型可能在糖酵解和抗氧化剂方面存在差异。
