Sun Wenchao, Cao Huihui, Zheng Min, Xu Suping, Zhang Hongyun, Wei Xiankai, Su Jiaoxu, He Jieyong
Bing Du Xue Bao. 2016 Jul;32(4):429-35.
Dog circovirus (DogCV) is a newly discovered mammalian circovirus. To investigate the genomic characteristics and genetic diversity of DogCV spreads in China, the first genome sequence of Chinese isolate, designated as JZ98/2014,was obtained by overlap PCR using the DNA extracted from dog serum as template for amplification. The nucleotide content and genome organization were subsequently analyzed. The results showed that the full-length genome of JZ98/2014 is 2063nt,and contains three major open reading frame: ORF V1 (encodes the 303 amino acid Rep protein),ORF C1(encodes the 270 amino acid Cap protein),and ORF C2(encodes 106 amino acids).JZ98/2014 shared 82.1%-89.5% homology with the complete genome sequences of DogCV isolates from America and Europe. The Rep gene and Cap gene of JZ98/2014 shared 82.1%-89.5%and 84.6%-89.1% homology, respectively, with the same genes from other DogCVs. Phylogenetic tree analysis indicated that there were several different genetic clades of DogCV spread in the world, and JZ98/2014 formed a clade by itself.
犬圆环病毒（DogCV）是一种新发现的哺乳动物圆环病毒。为了研究在中国传播的犬圆环病毒的基因组特征和遗传多样性，以犬血清提取的DNA为模板，通过重叠PCR获得了中国分离株的首个基因组序列，命名为JZ98/2014。随后对其核苷酸含量和基因组结构进行了分析。结果显示，JZ98/2014的全长基因组为2063nt，包含三个主要开放阅读框：ORF V1（编码303个氨基酸的Rep蛋白）、ORF C1（编码270个氨基酸的Cap蛋白）和ORF C2（编码106个氨基酸）。JZ98/2014与来自美国和欧洲的犬圆环病毒分离株的全基因组序列具有82.1%-89.5%的同源性。JZ98/2014的Rep基因和Cap基因与其他犬圆环病毒的相同基因分别具有82.1%-89.5%和84.6%-89.1%的同源性。系统发育树分析表明，世界上传播的犬圆环病毒存在几个不同的遗传分支，JZ98/2014自成一个分支。
