Blood Systems Research Institute, and Department of Laboratory Medicine, University of California, San Francisco, California, USA.
Emerg Infect Dis. 2013 Apr;19(4):534-41. doi: 10.3201/eid1904.121390.
We characterized the complete genome of a novel dog circovirus (DogCV) from the liver of a dog with severe hemorrhagic gastroenteritis, vasculitis, and granulomatous lymphadenitis. DogCV was detected by PCR in fecal samples from 19/168 (11.3%) dogs with diarrhea and 14/204 (6.9%) healthy dogs and in blood from 19/409 (3.3%) of dogs with thrombocytopenia and neutropenia, fever of unknown origin, or past tick bite. Co-infection with other canine pathogens was detected for 13/19 (68%) DogCV-positive dogs with diarrhea. DogCV capsid proteins from different dogs varied by up to 8%. In situ hybridization and transmission electron microscopy detected DogCV in the lymph nodes and spleens of 4 dogs with vascular compromise and histiocytic inflammation. The detection of a circovirus in tissues of dogs expands the known tropism of these viruses to a second mammalian host. Our results indicate that circovirus, alone or in co-infection with other pathogens, might contribute to illness and death in dogs.
我们从一只患有严重出血性胃肠炎、血管炎和肉芽肿性淋巴结炎的狗的肝脏中，对一种新型犬圆环病毒（DogCV）的全基因组进行了特征描述。通过 PCR 在 168 只腹泻犬的粪便样本中检测到了 DogCV，阳性率为 19/168（11.3%），在 204 只健康犬的粪便样本中检测到了阳性率为 14/204（6.9%），在 409 只患有血小板减少症和中性粒细胞减少症、不明原因发热或过去被蜱虫叮咬的犬的血液中检测到了阳性率为 19/409（3.3%）。在患有腹泻的 19 只 DogCV 阳性犬中，有 13 只（68%）检测到了其他犬病原体的合并感染。来自不同犬的 DogCV 衣壳蛋白的差异高达 8%。原位杂交和透射电子显微镜检测到 4 只血管功能障碍和组织细胞性炎症犬的淋巴结和脾脏中有 DogCV。在犬的组织中检测到圆环病毒，扩大了这些病毒对第二种哺乳动物宿主的已知嗜性。我们的研究结果表明，圆环病毒单独或与其他病原体合并感染，可能会导致犬的疾病和死亡。
