爱泼斯坦-巴尔病毒感染细胞中脱氧尿苷三磷酸核苷酸水解酶活性的诱导
Induction of a deoxyuridine triphosphate nucleotidohydrolase activity in Epstein-Barr virus-infected cells.
作者信息
Williams M V, Holliday J, Glaser R
出版信息
Virology. 1985 Apr 30;142(2):326-33. doi: 10.1016/0042-6822(85)90341-1.
Superinfection of Raji cells with Epstein-Barr virus (EBV) or chemical induction of HR-1 cells with 12-O-tetradecanoyl-phorbol-13-acetate (TPA) results in the induction of a deoxyuridine triphosphate nucleotidohydrolase (dUTPase) which is not observed in mock-treated cells or TPA-treated EBV genome-negative BJAB cells. The EBV-induced dUTPase could be distinguished from the host dUTPase based upon differences in their migration in polyacrylamide gels and sensitivity to the 5-mercurithioguanosine derivitive of dUTP. The expression of the EBV-specified dUTPase is prevented by phosphonoacetic acid indicating that its expression is dependent upon EBV-DNA replication.
用爱泼斯坦-巴尔病毒（EBV）对拉吉细胞进行双重感染，或用12-十四烷酰佛波醇-13-乙酸酯（TPA）对HR-1细胞进行化学诱导，结果会诱导出一种脱氧尿苷三磷酸核苷酸水解酶（dUTPase），而在模拟处理的细胞或TPA处理的EBV基因组阴性BJAB细胞中未观察到这种酶。基于EBV诱导的dUTPase在聚丙烯酰胺凝胶中的迁移差异及其对dUTP的5-巯基硫代鸟苷衍生物的敏感性，可以将其与宿主dUTPase区分开来。膦甲酸可阻止EBV特异性dUTPase的表达，这表明其表达依赖于EBV-DNA复制。
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