病毒非生产性Raji细胞中的爱泼斯坦-巴尔病毒特异性DNA聚合酶
Epstein-Barr virus-specific DNA polymerase in virus-nonproducer Raji cells.
作者信息
Ooka T, Lenoir G M, Decaussin G, Bornkamm G W, Daillie J
出版信息
J Virol. 1986 May;58(2):671-5. doi: 10.1128/JVI.58.2.671-675.1986.
Abstract
Virus-nonproducer Raji cells, when induced to early antigen synthesis by 12-O-tetradecanoyl-phorbol-13-acetate and sodium butyrate, showed an increase in DNA polymerase activity. This enzyme has the characteristics of a typical Epstein-Barr virus DNA polymerase with regard to chromatographical pattern and biological properties: it is eluted from DEAE-cellulose at 0.08 M NaCl, has a high salt resistance, is sensitive to phosphonoacetic acid and phosphonoformate, and shows a substrate preference for poly(dC)-oligo(dG12-18). The resistance of Epstein-Barr virus polymerase activity to aphidicolin is a property distinct from that of HSV DNA polymerase. Viral DNA polymerase activity increases in the absence of Epstein-Barr virus DNA replication, indicating that this enzyme is an early viral protein.
摘要
病毒非生产性拉吉细胞在被十四酰佛波醇-13-乙酸酯和丁酸钠诱导进行早期抗原合成时，其DNA聚合酶活性增加。就色谱模式和生物学特性而言，这种酶具有典型的爱泼斯坦-巴尔病毒DNA聚合酶的特征：它在0.08M NaCl浓度下从DEAE-纤维素上洗脱，具有高耐盐性，对膦乙酸和膦甲酸敏感，并且对聚（dC）-寡聚（dG12 - 18）表现出底物偏好。爱泼斯坦-巴尔病毒聚合酶活性对阿非科林的抗性是一种与单纯疱疹病毒DNA聚合酶不同的特性。在没有爱泼斯坦-巴尔病毒DNA复制的情况下，病毒DNA聚合酶活性增加，这表明该酶是一种早期病毒蛋白。
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