Sánchez A, Banerjee A K
Virology. 1985 May;143(1):45-54. doi: 10.1016/0042-6822(85)90095-9.
The structural proteins of human parainfluenza virus 3, a member of the paramyxovirus family, were characterized by SDS-polyacrylamide gel electrophoresis of radiolabeled virus. The purified virion contains at least eight structural proteins, with estimated molecular weights of 251K, 90K, 71K, 68K, 65K, 51K, 35K, and 21K, respectively. Three of the polypeptides (71K, 65K, and 51K) were identified as glycoproteins based on their incorporation of [3H]glucosamine. Disruption of the virus by Triton X-100 in the presence of increasing salt concentrations indicated that the polypeptides of molecular weights 251K, 90K, 68K, and 21K were components of the nucleocapsid. In parainfluenza virus 3 infected BS-C-1 cells, seven virus structural polypeptides were identified. Six structural proteins (90K, 71K, 68K, 51K, 35K, and 21K) were detected in the cell lysate at 7 hr after infection, while at 10 hr an additional polypeptide (251K) was also observed. At least two nonstructural polypeptides of molecular weights 30K and 25K were also detected in infected cells. mRNAs isolated from virus-infected cells were translated in a cell-free protein-synthesizing system. The in vitro translation products were identical to the authentic virion polypeptides as determined by partial digestion with staphylococcal V8 protease.
人副流感病毒3型属于副粘病毒科，其结构蛋白通过对放射性标记病毒进行SDS聚丙烯酰胺凝胶电泳来表征。纯化的病毒粒子含有至少八种结构蛋白，估计分子量分别为251K、90K、71K、68K、65K、51K、35K和21K。其中三种多肽（71K、65K和51K）基于其对[3H]葡糖胺的掺入被鉴定为糖蛋白。在盐浓度增加的情况下，用Triton X-100破坏病毒表明，分子量为251K、90K、68K和21K的多肽是核衣壳的组成部分。在感染了副流感病毒3型的BS-C-1细胞中，鉴定出了七种病毒结构多肽。感染后7小时，在细胞裂解物中检测到六种结构蛋白（90K、71K、68K、51K、35K和21K），而在10小时时还观察到另一种多肽（251K）。在感染细胞中还检测到至少两种分子量为30K和25K的非结构多肽。从病毒感染细胞中分离的mRNA在无细胞蛋白质合成系统中进行翻译。通过用葡萄球菌V8蛋白酶部分消化确定，体外翻译产物与真实的病毒粒子多肽相同。
