Hundley F, Biryahwaho B, Gow M, Desselberger U
Virology. 1985 May;143(1):88-103. doi: 10.1016/0042-6822(85)90099-6.
After serial passage at high multiplicity of infection of standard bovine rotavirus in MA104 cells, different genome rearrangements occurred in which segment 5 was lost from the RNA profile and distinct additional bands of double-stranded (ds) RNA were found in positions on gels between segments 1 and 6. It was shown that some of the additional RNA bands contained segment 5-specific sequences. The additional RNA bands were transcribed in vitro to apparent full length. Analysis of the proteins synthesized in cells infected with viruses possessing rearranged genomes showed that in all cases the product of RNA segment 5, VP5, was missing; however, in one case an abnormal protein was observed which corresponded in size to the coding capacity of the mRNA transcribed from the additional genomic RNA band. Viruses with rearranged genomes could be plaque purified, and they grew in the absence of standard virus to titers comparable to those obtained from standard virus. In mixed infections of standard virus and virus possessing genome rearrangements, standard virus overgrew during passage at low multiplicity of infection whereas virus possessing genome rearrangements overgrew during passage at high multiplicity of infection.
标准牛轮状病毒在MA104细胞中以高感染复数进行连续传代后，发生了不同的基因组重排，其中RNA图谱中第5节段缺失，在凝胶上第1节段和第6节段之间的位置发现了双链（ds）RNA的明显附加条带。结果表明，一些附加RNA条带含有第5节段特异性序列。附加RNA条带在体外转录至明显的全长。对感染了具有重排基因组病毒的细胞中合成的蛋白质进行分析表明，在所有情况下，RNA第5节段的产物VP5均缺失；然而，在一种情况下观察到一种异常蛋白质，其大小与从附加基因组RNA条带转录的mRNA的编码能力相符。具有重排基因组的病毒可以通过空斑纯化，并且它们在没有标准病毒的情况下生长，其滴度与从标准病毒获得的滴度相当。在标准病毒和具有基因组重排的病毒的混合感染中，标准病毒在低感染复数传代过程中过度生长，而具有基因组重排的病毒在高感染复数传代过程中过度生长。
