Samal S K, Dopazo C P, Subramanian K, Lupiani B, Mohanty S B, Hetrick F M
College of Veterinary Medicine, University of Maryland, College Park 20742.
J Gen Virol. 1991 Jan;72 ( Pt 1):181-4. doi: 10.1099/0022-1317-72-1-181.
Biochemical characteristics of five rotavirus-like viruses isolated from striped bass (Morone saxatilis), turbot (Scophthalmus maximus), smelt (Osmerus mordax) and Atlantic salmon (Salmo salar) in North America and Europe were compared. The genome of each isolate was composed of 11 segments of dsRNA and each isolate had a unique electropherotype in polyacrylamide gels. Agarose gel electrophoresis showed similar RNA profiles for all four isolates from North America, whereas the RNA profile of the isolate from Europe was different. Analysis of virion proteins revealed that each virus had five structural proteins ranging in Mr from 130,000 to 34,000. Each isolate had a unique polypeptide profile but their overall polypeptide patterns were similar. Reciprocal RNA-RNA blot hybridization demonstrated that all these rotavirus-like viruses cross-hybridized with each other except for the isolate from Europe which did not hybridize with the RNA from any of the other isolates. No genetic relationship was found between these rotavirus-like viruses of fish and a true group A rotavirus (SA11).
对从北美和欧洲的条纹鲈（Morone saxatilis）、大菱鲆（Scophthalmus maximus）、胡瓜鱼（Osmerus mordax）和大西洋鲑（Salmo salar）中分离出的五种轮状病毒样病毒的生化特性进行了比较。每个分离株的基因组由11个双链RNA片段组成，并且每个分离株在聚丙烯酰胺凝胶中都有独特的电泳图谱型。琼脂糖凝胶电泳显示，来自北美的所有四个分离株具有相似的RNA图谱，而来自欧洲的分离株的RNA图谱则不同。病毒粒子蛋白分析表明，每种病毒都有五种结构蛋白，其分子量在130,000至34,000之间。每个分离株都有独特的多肽图谱，但它们的总体多肽模式相似。相互的RNA-RNA印迹杂交表明，除了来自欧洲的分离株不与任何其他分离株的RNA杂交外，所有这些轮状病毒样病毒之间都相互交叉杂交。在这些鱼类轮状病毒样病毒与真正的A组轮状病毒（SA11）之间未发现遗传关系。
