Kang C Y, Schubert M, Lazzarini R A
Virology. 1985 Jun;143(2):630-5. doi: 10.1016/0042-6822(85)90403-9.
We have isolated and partially characterized a number of different genome types of defective interfering (DI) particles newly generated by a highly heat-resistant strain of vesicular stomatitis virus in either Rat(B77) or Vero cells. Northern blot analyses revealed that many of these DI genomes contain N gene sequences and/or sequences of the NS, M, and G genes. One type contains NS sequences without any indication for the presence of either N, M, or G sequences. Another type of DI particle genomes did not contain any detectable sequences of N, NS, M, or G, but contain panhandle-type sequences and, thus, most likely resembles the 5'-panhandle-type DI particles. Unlike previously assumed, these data demonstrate that DI genomes which have the 3'-terminal N, NS, M, and G genes or portions of these genes conserved do frequently arise together with 5'-DI particle genomes after serial undiluted passages of the heat-resistant strain of vesicular stomatitis virus.
我们已经分离并部分鉴定了由水疱性口炎病毒的一种高度耐热菌株在大鼠（B77）或非洲绿猴肾细胞（Vero细胞）中新产生的多种不同基因组类型的缺陷干扰（DI）颗粒。Northern印迹分析表明，这些DI基因组中的许多都包含N基因序列和/或NS、M和G基因的序列。一种类型包含NS序列，但没有任何迹象表明存在N、M或G序列。另一种类型的DI颗粒基因组不包含N、NS、M或G的任何可检测序列，但包含柄型序列，因此很可能类似于5'-柄型DI颗粒。与之前的假设不同，这些数据表明，在水疱性口炎病毒耐热菌株连续未稀释传代后，具有3'-末端N、NS、M和G基因或这些基因部分保守的DI基因组确实经常与5'-DI颗粒基因组一起出现。
