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具有不同5'-末端互补量的水疱性口炎病毒缺陷干扰基因组的分离

Isolation of vesicular stomatitis virus defective interfering genomes with different amounts of 5'-terminal complementarity.

作者信息

Kolakofsky D

出版信息

J Virol. 1982 Feb;41(2):566-74. doi: 10.1128/JVI.41.2.566-574.1982.

DOI:10.1128/JVI.41.2.566-574.1982
PMID:6281468
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC256785/
Abstract

I isolated at least 30 different vesicular stomatitis virus defective interfering (DI) genomes, distinguished by chain length, by five independent undiluted passages of a repeatedly cloned virus plaque. Labeling of the 3' hydroxyl ends of these DI genomes and RNase digestion studies demonstrated that the ends of these DI genomes were terminally complementary to different extents (approximately 46 to 200 nucleotides). Mapping studies showed that the complementary ends of all of the DI genomes were derived from the 5' ends of the nondefective minus-strand genome. Regardless of the extent of terminal complementarity, all of the DI genomes synthesized the same 46-nucleotide minus-strand leader RNA.

摘要

我通过对一个反复克隆的病毒蚀斑进行五次独立的未稀释传代，分离出了至少30种不同的水泡性口炎病毒缺陷干扰（DI）基因组，这些基因组通过链长来区分。对这些DI基因组的3'羟基末端进行标记以及核糖核酸酶消化研究表明，这些DI基因组的末端在不同程度上（约46至200个核苷酸）存在末端互补。图谱研究表明，所有DI基因组的互补末端均来自非缺陷负链基因组的5'末端。无论末端互补的程度如何，所有DI基因组都合成相同的46个核苷酸的负链前导RNA。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8453/256785/4c805a454573/jvirol00161-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8453/256785/5beee35f8414/jvirol00161-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8453/256785/88cb0c4f0abc/jvirol00161-0225-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8453/256785/a1d68bc99851/jvirol00161-0226-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8453/256785/3fe74fc5dcca/jvirol00161-0226-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8453/256785/3e5ea20d42cb/jvirol00161-0227-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8453/256785/4c805a454573/jvirol00161-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8453/256785/5beee35f8414/jvirol00161-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8453/256785/88cb0c4f0abc/jvirol00161-0225-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8453/256785/a1d68bc99851/jvirol00161-0226-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8453/256785/3fe74fc5dcca/jvirol00161-0226-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8453/256785/3e5ea20d42cb/jvirol00161-0227-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8453/256785/4c805a454573/jvirol00161-0228-a.jpg

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Isolation of vesicular stomatitis virus defective interfering genomes with different amounts of 5'-terminal complementarity.具有不同5'-末端互补量的水疱性口炎病毒缺陷干扰基因组的分离
J Virol. 1982 Feb;41(2):566-74. doi: 10.1128/JVI.41.2.566-574.1982.
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J Virol. 1982 Jan;41(1):210-21. doi: 10.1128/JVI.41.1.210-221.1982.
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Effect of defective interfering particles on plus- and minus- strand leader RNAs in vesicular stomatitis virus-infected cells.缺陷干扰颗粒对感染水疱性口炎病毒的细胞中正链和负链前导RNA的影响。
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引用本文的文献

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Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8587-91. doi: 10.1073/pnas.91.18.8587.
2
Structure and origin of a novel class of defective interfering particle of vesicular stomatitis virus.水泡性口炎病毒一类新型缺陷干扰颗粒的结构与起源
Nucleic Acids Res. 1984 Mar 26;12(6):2775-90. doi: 10.1093/nar/12.6.2775.
3
Sequence determination of the (+) leader RNA regions of the vesicular stomatitis virus Chandipura, Cocal, and Piry serotype genomes.

本文引用的文献

1
Vesicular stomatitis virus defective interfering particle containing a muted internal leader RNA gene.含有沉默内部引导RNA基因的水疱性口炎病毒缺陷干扰颗粒。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2090-4. doi: 10.1073/pnas.78.4.2090.
2
In vivo transcription of the 5'-terminal extracistronic region of vesicular stomatitis virus RNA.水疱性口炎病毒RNA 5'端顺反子外区域的体内转录
J Virol. 1981 Apr;38(1):256-62. doi: 10.1128/JVI.38.1.256-262.1981.
3
Interaction of VSV leader RNA and nucleocapsid protein may control VSV genome replication.
水疱性口炎病毒钱迪普拉、科卡尔和皮里血清型基因组（+）前导RNA区域的序列测定。
J Virol. 1983 Apr;46(1):125-30. doi: 10.1128/JVI.46.1.125-130.1983.
水泡性口炎病毒前导RNA与核衣壳蛋白的相互作用可能控制水泡性口炎病毒基因组的复制。
Cell. 1981 Mar;23(3):837-45. doi: 10.1016/0092-8674(81)90448-7.
4
Comparison of ribonucleotide sequences from the genome of vesicular stomatitis virus and two of its defective interfering particles.水疱性口炎病毒基因组及其两个缺陷干扰颗粒的核糖核苷酸序列比较。
J Virol. 1981 Jan;37(1):363-71. doi: 10.1128/JVI.37.1.363-371.1981.
5
Effect of defective interfering particles on plus- and minus- strand leader RNAs in vesicular stomatitis virus-infected cells.缺陷干扰颗粒对感染水疱性口炎病毒的细胞中正链和负链前导RNA的影响。
J Virol. 1980 Sep;35(3):704-9. doi: 10.1128/JVI.35.3.704-709.1980.
6
Utilization of the guanylyltransferase and methyltransferases of vaccinia virus to modify and identify the 5'-terminals of heterologous RNA species.利用痘苗病毒的鸟苷酸转移酶和甲基转移酶修饰和鉴定异源RNA物种的5'-末端。
Biochem Biophys Res Commun. 1977 Jan 24;74(2):374-83. doi: 10.1016/0006-291x(77)90314-x.
7
3'-terminal labelling of RNA with T4 RNA ligase.利用T4 RNA连接酶对RNA进行3'末端标记
Nature. 1978 Oct 12;275(5680):560-1. doi: 10.1038/275560a0.
8
Defective interfering particles of rhabdoviruses.弹状病毒的缺陷干扰颗粒
Curr Top Microbiol Immunol. 1979;86:123-68. doi: 10.1007/978-3-642-67341-2_4.
9
A new method for sequencing DNA.一种新的DNA测序方法。
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4. doi: 10.1073/pnas.74.2.560.
10
A specific internal RNA polymerase recognition site of VSV RNA is involved in the generation of DI particles.水疱性口炎病毒（VSV）RNA的一个特定内部RNA聚合酶识别位点参与了缺陷干扰颗粒的产生。
Cell. 1979 Nov;18(3):749-57. doi: 10.1016/0092-8674(79)90128-4.