Yamagishi M, Fujisawa H, Minagawa T
Virology. 1985 Jul 30;144(2):502-15. doi: 10.1016/0042-6822(85)90290-9.
In vitro DNA-packaging systems of bacteriophages T3 and T7 packaged homologous DNA more efficiently than heterologous DNA. Packaging of phage DNA proceeds by way of concatemeric intermediates (H. Fujisawa, J. Miyazaki, and T. Minagawa (1978), Virology 87, 394-400). The conversion of mature homologous and heterologous DNAs to concatemers was efficient in both the T3- and T7-packaging systems. In vitro complementation experiments indicate that the gene 19 product (gp19) specifies which DNA enters the capsid. To identify DNA regions recognized by the packaging systems, T3/T7 hybrids were constructed and physical maps of the hybrid DNAs were determined by restriction enzyme analysis. By comparing restriction maps and in vitro packaging of hybrid DNAs, it is concluded that the sequence responsible for specificity of DNA packaging is confined within 5% of the ends of the T3 and T7 genomes.
噬菌体T3和T7的体外DNA包装系统包装同源DNA比异源DNA更有效。噬菌体DNA的包装通过串联中间体进行（藤泽浩、宫崎纯司和皆川敏郎（1978年），《病毒学》87卷，394 - 400页）。在T3和T7包装系统中，成熟的同源和异源DNA转化为串联体的效率都很高。体外互补实验表明，基因19产物（gp19）决定了哪种DNA进入衣壳。为了鉴定包装系统识别的DNA区域，构建了T3/T7杂种，并通过限制性酶切分析确定了杂种DNA的物理图谱。通过比较杂种DNA的限制性图谱和体外包装情况，得出结论：负责DNA包装特异性的序列局限于T3和T7基因组末端的5%范围内。
