Compton T, Courtney R J
Virology. 1985 Nov;147(1):1-8. doi: 10.1016/0042-6822(85)90221-1.
Incorporation of [3H]mannose into cellular pools of mannosylphosphoryl dolichol (Man-P-Dol), oligosaccharide-lipid, and glycoprotein was measured and compared in herpes simplex virus type 1 (HSV-1)-infected cells and -uninfected cells. While mannose incorporation into the monosaccharide-dolichol fraction was similar in infected or uninfected Vero cells, incorporation into the oligosaccharide-lipid fraction was markedly reduced in HSV-1-infected cells (64% of control levels). In contrast, mannose incorporation into glycoprotein was significantly increased in virus-infected cells versus uninfected cells (194% of control levels). The kinetics of incorporation into the various fractions was examined and it was determined that there was minimal increase in mannose incorporation into oligosaccharide-lipid after 8 hr postinfection in virus-infected cells. This corresponded to the time at which nonglycosylated precursors of the HSV-1 glycoproteins were first detected in association with the nuclear fraction. These data suggest that there is an accelerated turnover of oligosaccharide-lipid in virus-infected Vero cells which is most likely due to extensive glycoprotein synthesis.
在单纯疱疹病毒1型（HSV-1）感染的细胞和未感染的细胞中，测定并比较了[3H]甘露糖掺入甘露糖基磷酸多萜醇（Man-P-Dol）、寡糖脂和糖蛋白的细胞池中的情况。虽然在感染或未感染的Vero细胞中，甘露糖掺入单糖-多萜醇部分的情况相似，但在HSV-1感染的细胞中，掺入寡糖脂部分的情况明显减少（为对照水平的64%）。相反，与未感染的细胞相比，病毒感染的细胞中甘露糖掺入糖蛋白的情况显著增加（为对照水平的194%）。研究了掺入各部分的动力学，确定在病毒感染的细胞中感染后8小时，甘露糖掺入寡糖脂的增加最小。这与首次在核部分检测到HSV-1糖蛋白的非糖基化前体的时间一致。这些数据表明，病毒感染的Vero细胞中寡糖脂的周转加速，这很可能是由于广泛的糖蛋白合成所致。
