Sommer M, Courtney R J
Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, Shreveport 71130-3932.
J Virol. 1991 Jan;65(1):520-5. doi: 10.1128/JVI.65.1.520-525.1991.
The kinetics of processing and transport of herpes simplex virus type 1 (HSV-1) glycoproteins gB and gC was investigated. The conversion of precursor to mature forms and the appearance of the glycoproteins at the infected-cell surface at different times postinfection (p.i.) were studied. gB, synthesized at 4 h p.i., was converted to the mature form with a half-time (t1/2) of 120 min and appeared at the plasma membrane with a t1/2 of 270 min. The gB synthesized at later times p.i. (6, 8, and 10.5 h) was transported less efficiently. Less than 50% of gB synthesized at later times p.i. was processed and transported to the cell surface. gB synthesized in transfected cells was transported to the plasma membrane with kinetics similar to that for gB synthesized at early times p.i. gC was processed efficiently when synthesized at both 8 and 10.5 h p.i., with t1/2 of conversion of pgC to gC of 40 and 60 min, respectively. Approximately 90 to 95% of the gC synthesized was converted to the mature form. The gC synthesized at 8 h p.i. was also transported rapidly to the cell surface, compared with the transport of gB synthesized at the same time, with a t1/2 of 240 min. Greater than 70% of the gC synthesized at 8 h p.i. appeared at the cell surface. The gC synthesized at 10.5 h was transported less efficiently to the cells surface during a 6-h chase.
对单纯疱疹病毒1型（HSV-1）糖蛋白gB和gC的加工及转运动力学进行了研究。研究了感染后不同时间（p.i.）前体向成熟形式的转化以及糖蛋白在感染细胞表面的出现情况。感染后4小时合成的gB以120分钟的半衰期（t1/2）转化为成熟形式，并以270分钟的t1/2出现在质膜上。感染后较晚时间（6、8和10.5小时）合成的gB转运效率较低。感染后较晚时间合成的gB中，不到50%被加工并转运到细胞表面。在转染细胞中合成的gB以与感染后早期合成的gB相似的动力学转运到质膜。当在感染后8小时和10.5小时合成时，gC被有效加工，pgC向gC转化的t1/2分别为40分钟和60分钟。合成的gC中约90%至95%转化为成熟形式。与同时合成的gB的转运相比，感染后8小时合成的gC也迅速转运到细胞表面，t1/2为240分钟。感染后8小时合成的gC中超过70%出现在细胞表面。在6小时的追踪过程中，感染后10.5小时合成的gC转运到细胞表面的效率较低。
