Rhoo Kun Hyoe, Robert Jacques
Department of Immunology Microbiology and Virology, University of Rochester, Medical Center, New York 14620
Department of Immunology Microbiology and Virology, University of Rochester, Medical Center, New York 14620.
Cold Spring Harb Protoc. 2019 May 1;2019(5):pdb.prot097592. doi: 10.1101/pdb.prot097592.
Adoptive cell transfer from inbred adult to inbred tadpoles is a useful way to study the dissemination of immune cells or pathogen-infected immune cells in tadpoles. For example, peritoneal leukocytes (PLs) can be readily infected by pathogens such as Frog virus 3 (FV3) and (). By transferring fluorescently labeled, FV3-infected PLs into tadpoles, we observed infiltration of these cells into the tadpole's brain, which indicates that FV3-infected PLs can cross blood brain barrier. Taking advantage of tadpoles' transparency, fluorescently labeled immune cells can be tracked in real time using fluorescence microscopy.
将近交系成年动物的免疫细胞转移至近交系蝌蚪是研究免疫细胞或病原体感染的免疫细胞在蝌蚪体内扩散的一种有用方法。例如，腹膜白细胞（PLs）很容易被诸如蛙病毒3（FV3）等病原体感染。通过将荧光标记的、被FV3感染的PLs转移到蝌蚪体内，我们观察到这些细胞浸润到蝌蚪的大脑中，这表明被FV3感染的PLs能够穿越血脑屏障。利用蝌蚪的透明性，可以使用荧光显微镜实时追踪荧光标记的免疫细胞。
