Department of Electricity, Solid Physics and Biophysics, Faculty of Physics, University of Bucharest, Str. Atomistilor no. 405, CP MG - 11, Bucuresti-Magurele RO 077125, Romania.
Department of Life and Environmental Physics, "Horia Hulubei" National Institute of Physics and Nuclear Engineering, Str. Reactorului no. 30, P.O. BOX MG-6, Bucharest-Magurele, Romania.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Nov 5;204:648-656. doi: 10.1016/j.saa.2018.06.093. Epub 2018 Jun 28.
Folic acid (FA) is a water soluble bioactive food constituent from the vitamin B-family complex (B9). FA deficiency can lead to a variety of human health problems, while a high intake of FA can reduce the cytotoxicity of natural killer cells. The main goal of this study was to investigate the interaction of FA with human serum albumin (HSA) at physiological pH using ATR-FTIR, fluorescence spectroscopy, cyclic voltammetry and electrochemical impedance spectroscopy in order to understand the role of HSA as a blood transporter for FA in aqueous solution that can be used in different therapies. The quenching of HSA in the presence of FA was followed and the binding constant (K) was determined. The variation of electrochemical parameters proved that the FA binds to immobilized HSA and the binding constant was ten times than the value obtained when the interaction takes place between free molecules in solution when studied by fluorescence quenching. The results can be used in future studies to improve drug delivery systems or cellular uptake of folic acid and food components conjugated to HSA nanoparticles or nanocapsules.
叶酸（FA）是一种水溶性生物活性食品成分，属于维生素 B 族复合物（B9）。FA 缺乏会导致多种人类健康问题，而高摄入量的 FA 可以降低自然杀伤细胞的细胞毒性。本研究的主要目的是使用 ATR-FTIR、荧光光谱法、循环伏安法和电化学阻抗谱法在生理 pH 值下研究 FA 与人血清白蛋白（HSA）之间的相互作用，以了解 HSA 作为 FA 在水溶液中的血液转运蛋白的作用，可用于不同的治疗方法。在存在 FA 的情况下，跟踪 HSA 的猝灭，并确定结合常数（K）。电化学参数的变化证明 FA 与固定化 HSA 结合，并且结合常数是通过荧光猝灭研究溶液中游离分子之间相互作用时获得的值的十倍。这些结果可用于未来的研究，以改善叶酸和与 HSA 纳米颗粒或纳米胶囊缀合的食物成分的药物传递系统或细胞摄取。
