Asfandiiarov R I, Volodin M A, Kozlov S V, Laz'ko A E
Arkh Anat Gistol Embriol. 1985 Sep;89(9):74-80.
By means of histochemical techniques at light and electron microscopic levels, as well as immunomorphological, biochemical and immunochemical methods localization and dynamics of alkaline phosphatase and 5'-nucleotidase contents have been determined in anlages of long tubular bones in 85 human embryos and prefetuses from the 6th up to 12th week of the intrauterine development, obtained as a result of artificial abortions in healthy women. The greatest activity of the enzymes studied is revealed in areas of an intensive osteogenesis and mineralization. Also, by means of the immunofluorescent method alkaline phosphatase of a placental type is revealed, that is not revealed, however, immunochemically. With increasing time of the intrauterine development, thermostability of alkaline phosphatase increases.
通过光镜和电镜水平的组织化学技术，以及免疫形态学、生物化学和免疫化学方法，对85例健康女性人工流产获得的妊娠6至12周的人类胚胎和胎儿长管状骨原基中碱性磷酸酶和5'-核苷酸酶含量的定位及动态变化进行了测定。在所研究的酶中，最大活性出现在成骨和矿化活跃的区域。此外，通过免疫荧光法发现了胎盘型碱性磷酸酶，但免疫化学方法未检测到。随着宫内发育时间的增加，碱性磷酸酶的热稳定性增强。
