从 2011 年至 2016 年住院患者尿液样本中分离的近平滑假丝酵母菌的基于序列的鉴定、基因分型和毒力因子。
Sequence-based identification, genotyping and virulence factors of Trichosporon asahii strains isolated from urine samples of hospitalized patients (2011-2016).
机构信息
Hacettepe University Faculty of Medicine, Department of Medical Microbiology, Hacettepe Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji ABD, Sıhhiye, 06100 Ankara, Turkey.
Selçuk Üniversity Medicine Faculty, Department of Medical Genetics, Selçuk Üniversitesi Tıp Fakültesi Tıbbi Genetik ABD Selçuklu/Konya, Ankara, Turkey.
出版信息
J Mycol Med. 2018 Sep;28(3):452-456. doi: 10.1016/j.mycmed.2018.06.006. Epub 2018 Jul 6.
INTRODUCTION
Trichosporon asahii is the most common species that causes trichosporonosis.
MATERIALS AND METHODS
In the present study, a collection of 68T. asahii strains recovered from hospitalized patients urine samples between 2011 and 2016 was examined. T. asahii strains were identified by sequencing the intergenic spacer 1 region (IGS1) and genotyped. In addition, proteinase, phospholipase, esterase, haemolytic activity, and biofilm formation of a total of T. asahii strains were investigated.
RESULTS
The predominant genotype was 1 (79.3%) and followed by 5 (8%), 3 (6.9%), 6 (3.4%), 4 (1.1%), 9 (1.1%). In none of the 68 strains, proteinase and phospholipase activities could be detected, while all were found to be esterase positive. Biofilm production and hemolytic activity were detected in 23.5 and 97% respectively.
DISCUSSION
Our results indicated that six genotypes were (1, 5, 3, 6, 4, 9) present among T. asahii strains and no property was found to associate with a genotype, in terms of virulence factors.
简介
解脂假丝酵母是引起假丝酵母菌病最常见的物种。
材料与方法
本研究对 2011 年至 2016 年间从住院患者尿液样本中分离出的 68 株解脂假丝酵母菌株进行了检测。通过对基因间隔区 1 区（IGS1）进行测序和基因分型来鉴定解脂假丝酵母菌株。此外，还对所有解脂假丝酵母菌株的蛋白酶、磷脂酶、酯酶、溶血活性和生物膜形成能力进行了研究。
结果
主要基因型为 1（79.3%），其次为 5（8%）、3（6.9%）、6（3.4%）、4（1.1%）、9（1.1%）。在这 68 株菌株中，均未检测到蛋白酶和磷脂酶活性，但所有菌株均为酯酶阳性。生物膜生成和溶血活性的检出率分别为 23.5%和 97%。
讨论
我们的结果表明，6 种基因型（1、5、3、6、4、9）存在于解脂假丝酵母菌株中，在毒力因子方面，未发现任何特性与基因型相关。
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