一种分离钙蛋白酶I、钙蛋白酶II和钙蛋白酶抑制蛋白的简单一步法。
A simple one-step procedure for the separation of calpain I, calpain II and calpastatin.
作者信息
Karlsson J O, Gustavsson S, Hall C, Nilsson E
出版信息
Biochem J. 1985 Oct 1;231(1):201-4. doi: 10.1042/bj2310201.
Abstract
The soluble fraction from rabbit brain was adsorbed on a column of phenyl-Sepharose. By applying a linear gradient with decreasing salt concentration and increasing pH, it was possible to separate calpain I and calpain II from each other and from the endogenous inhibitor calpastatin. Both enzymes were capable of degrading endogenously labelled neuronal proteins, including slowly axonally transported soluble proteins and rapidly transported membrane-bound proteins, as well as casein.
摘要
兔脑可溶性组分吸附于苯基琼脂糖柱上。通过施加盐浓度降低和pH升高的线性梯度，可将钙蛋白酶I和钙蛋白酶II彼此分离，并与内源性抑制剂钙蛋白酶抑制蛋白分离。这两种酶都能够降解内源性标记的神经元蛋白，包括缓慢轴突运输的可溶性蛋白和快速运输的膜结合蛋白，以及酪蛋白。
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