Westphal M, Harsh G R, Rosenblum M L, Hammonds R G
Biochem Biophys Res Commun. 1985 Oct 15;132(1):284-9. doi: 10.1016/0006-291x(85)91020-4.
Two established human tumor cell lines, epidermoid carcinoma line A431 and glioblastoma line SF268, were studied to compare the interaction of each with epidermal growth factor (EGF). SF268 cells bound [125I] EGF with 35-40 fold higher affinity than did the A431 cells. The EGF binding sites of both lines were photoaffinity labeled using 2,4-NAPS-[125I] EGF, a photoreactive derivative of EGF. Extracts of photolysed cells analyzed by SDS-PAGE showed a difference between the two cell lines in the high molecular weight component corresponding to the EGF receptor. EGF in a dose range from 0.3-200 nM had no effect on thymidine incorporation by SF268 cells, whereas thymidine incorporation by A431 cells was markedly inhibited by EGF.
研究了两种成熟的人类肿瘤细胞系，即表皮样癌细胞系A431和成胶质细胞瘤细胞系SF268，以比较它们各自与表皮生长因子（EGF）的相互作用。SF268细胞结合[125I] EGF的亲和力比A431细胞高35 - 40倍。使用EGF的光反应性衍生物2,4-NAPS-[125I] EGF对这两种细胞系的EGF结合位点进行光亲和标记。通过SDS-PAGE分析光裂解细胞的提取物，结果显示在对应于EGF受体的高分子量组分上，这两种细胞系存在差异。浓度范围为0.3 - 200 nM的EGF对SF268细胞的胸苷掺入没有影响，而A431细胞的胸苷掺入则受到EGF的显著抑制。
