Harsh G R, Rosenblum M L, Williams L T
Department of Neurological Surgery, School of Medicine, University of California, San Francisco.
J Neurooncol. 1989 May;7(1):47-56. doi: 10.1007/BF00149378.
Oncogenes induce malignant transformation of cells. Two oncogenes are closely related to genes coding for a mitogenic growth factor (v-sis to the PDGF gene) and a receptor for a mitogenic growth factor (v-erb B to the EGF receptor gene). We studied the possibility that cells derived from malignant gliomas produce mitogenic factors that bind to cell surface receptors, the activation of which could lead to excessive stimulation of cell proliferation. All six cell lines tested secrete into their medium factors that stimulate DNA synthesis. The factor secreted by one cell line was characterized and found to resemble PDGF. Six of 11 cell lines had receptors for PDGF demonstrable by binding and receptor autophosphorylation assays. Six of six cell lines tested had EGF receptors demonstrable by binding and receptor autophosphorylation experiments. The extremely high levels of EGF receptor in one cell line may reflect excessive expression of the erb B oncogene associated with abnormalities of chromosome 7 that occur in this cell line.
癌基因可诱导细胞发生恶性转化。有两种癌基因分别与编码促有丝分裂生长因子的基因（v-sis与血小板衍生生长因子基因相关）和促有丝分裂生长因子的受体基因（v-erb B与表皮生长因子受体基因相关）密切相关。我们研究了恶性胶质瘤来源的细胞产生能与细胞表面受体结合的促有丝分裂因子的可能性，这种受体的激活可能导致细胞增殖的过度刺激。所检测的所有六种细胞系均向培养基中分泌刺激DNA合成的因子。对其中一个细胞系分泌的因子进行了特性分析，发现其类似于血小板衍生生长因子。通过结合和受体自磷酸化试验，11个细胞系中有6个具有血小板衍生生长因子受体。通过结合和受体自磷酸化实验，所检测的6个细胞系均有表皮生长因子受体。一个细胞系中表皮生长因子受体水平极高，这可能反映了与该细胞系中发生的7号染色体异常相关的erb B癌基因的过度表达。
