Activators of protein kinase C stimulate meiotic maturation of rat oocytes.

Agonistic analogs of gonadotropin releasing hormone can induce oocyte maturation in rat follicle-enclosed oocytes (1-5). Cyclic AMP does not rise following exposure of the ovarian follicle to GnRH (3) suggesting that cAMP-dependent protein kinase is not involved in the mechanism of GnRH action in this system. Protein kinase C, which is independent of cAMP, has recently been reported to mediate GnRH action in the pituitary (6-8). The possible involvement of this enzyme in the regulation of oocyte maturation has been tested in the present study. We report here that phospholipase C and direct activators of protein kinase C can mimic the response of rat oocytes to GnRH. These results suggest that GnRH-induced meiotic maturation of rat oocytes is mediated by the phospholipid-dependent protein kinase, protein kinase C.