环磷酸腺苷（cAMP）和蛋白激酶C对猪卵母细胞减数分裂成熟及丝裂原活化蛋白激酶（MAP激酶）磷酸化的抑制作用。
Inhibitory effects of cAMP and protein kinase C on meiotic maturation and MAP kinase phosphorylation in porcine oocytes.
作者信息
Fan Heng-Yu, Li Man-Yu, Tong Chao, Chen Da-Yuan, Xia Guo-Liang, Song Xiang-Fen, Schatten Heide, Sun Qing-Yuan
机构信息
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, People's Republic of China.
出版信息
Mol Reprod Dev. 2002 Dec;63(4):480-7. doi: 10.1002/mrd.10194.
The regulation of MAP kinase phosphorylation by cAMP and protein kinase C (PKC) modulators during pig oocyte maturation was studied by Western immunoblotting. We showed that both forskolin and IBMX inhibited MAP kinase phosphorylation and meiosis resumption in a dose-dependent manner, and this inhibitory effect was overcome by the protein phosphatase inhibitor, okadaic acid. Pharmacological PKC activator phorbol myristate acetate or physiological PKC activator diC8 also delayed MAP kinase phosphorylation and meiosis resumption, and their effect was abrogated by PKC inhibitors, staurosporine, and calphostin C. The results suggest that meiotic resumption is inhibited by elevation of cAMP or delayed by activation of PKC probably via down-regulation of MAP kinase activation, which is mediated by protein phosphatase, during pig oocyte maturation.
通过蛋白质免疫印迹法研究环磷酸腺苷（cAMP）和蛋白激酶C（PKC）调节剂在猪卵母细胞成熟过程中对丝裂原活化蛋白激酶（MAP激酶）磷酸化的调节作用。我们发现，福斯高林和异丁基甲基黄嘌呤（IBMX）均以剂量依赖的方式抑制MAP激酶磷酸化和减数分裂恢复，并且这种抑制作用可被蛋白磷酸酶抑制剂冈田酸所克服。药理学PKC激活剂佛波酯肉豆蔻酸酯乙酸盐或生理性PKC激活剂二辛酰甘油（diC8）也会延迟MAP激酶磷酸化和减数分裂恢复，其作用可被PKC抑制剂星形孢菌素和钙磷蛋白C消除。结果表明，在猪卵母细胞成熟过程中，减数分裂恢复可能通过蛋白磷酸酶介导的MAP激酶活化下调，被cAMP升高所抑制或被PKC激活所延迟。
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