小鼠原癌基因myb的结构组织
Structural organization of the mouse proto-myb gene.
作者信息
Castle S, Sheiness D
出版信息
Biochem Biophys Res Commun. 1985 Oct 30;132(2):688-95. doi: 10.1016/0006-291x(85)91187-8.
Proto-myb is a highly conserved cellular gene that is closely related to v-myb, the transforming gene of the avian myeloblastosis virus. We have isolated lambda clones encompassing 21 kbp of mouse DNA that contains portions of the proto-myb gene. Also, we have isolated a cDNA clone containing a 2.5 kbp insert corresponding to mouse proto-myb mRNA. By analyzing both the cloned DNAs and mouse genome DNA, we have constructed a restriction map for mouse proto-myb that extends over 50 kbp.
原癌基因myb是一种高度保守的细胞基因，与禽成髓细胞瘤病毒的转化基因v-myb密切相关。我们分离出了包含21kbp小鼠DNA的λ克隆，该DNA包含原癌基因myb的部分序列。此外，我们还分离出了一个cDNA克隆，其插入片段为2.5kbp，与小鼠原癌基因myb的mRNA相对应。通过分析克隆的DNA和小鼠基因组DNA，我们构建了一个覆盖超过50kbp的小鼠原癌基因myb的限制性图谱。
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