双光子光转化的毫秒级细胞标记
Millisecond cellular labelling with two-photon photoconversion.
作者信息
Kwok Sheldon J J, Jo Yongjae, Lin Harvey H, Choi Myunghwan, Yun Seok-Hyun
机构信息
Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 50 Blossom Street, Boston, MA 02114, USA.
Harvard-MIT Health Sciences and Technology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
出版信息
Biomed Opt Express. 2018 Jun 11;9(7):3067-3077. doi: 10.1364/BOE.9.003067. eCollection 2018 Jul 1.
labeling of cells within living biological tissues using photoconversion has provided valuable information on cellular physiology in their natural environments. However, current photoconvertible probes typically require seconds to minutes of light exposure, limiting their uses in rapid biological processes such as intracellular diffusion and circulating cells. Here, we report that two-photon photoconversion of cyanine-based dyes offers unprecedentedly rapid photoconversion down to millisecond timescales per cell. We demonstrate potential biological applications including measuring intracellular diffusion kinetics in a spinal nerve, labeling of rapidly flowing cells in a microfluidic channel, and photoconversion of a circulating cell .
利用光转化对活生物组织内的细胞进行标记，已为细胞在其自然环境中的生理学提供了有价值的信息。然而，目前的光转化探针通常需要数秒到数分钟的光照，这限制了它们在快速生物过程中的应用，如细胞内扩散和循环细胞。在此，我们报告基于菁染料的双光子光转化可实现前所未有的快速光转化，每个细胞的时间尺度可达毫秒级。我们展示了其潜在的生物学应用，包括测量脊髓神经中的细胞内扩散动力学、标记微流控通道中快速流动的细胞以及对循环细胞进行光转化。
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