Chen Jin, Wang Xiaoqian, Ghulam Murtaza, Chen Hongxu, Qu Feng
School of Life Science, Beijing Institute of Technology, Beijing, P. R. China.
Electrophoresis. 2018 Oct;39(19):2391-2397. doi: 10.1002/elps.201800154. Epub 2018 Aug 8.
In common partial filling CE (PF-CE), the capillary contains the selectors plug between the injection and detector end to avoid the selector going into the detector zone. To expand this method, we propose a mode of two discontinuous function plugs coupling in-capillary, named as plug-plug PF-CE (ppPF-CE). Initially, we present the method to predefine the effective length of chiral selector to meet the requirement of enantiomers' resolution, which could avoid some experimental procedures. With α-CD as a chiral selector, a satisfactory resolution of enantiomers d,l-tryptophan and d,l-tyrosine was obtained with a partial filling α-CD plug of optimal length and concentration. Subsequently, a second plug containing hydroxypropyl methylcellulose, organic solvents (acetonitrile and methanol), anionic and cationic surfactants (SDS and CTAB), and different concentrations of sodium phosphate buffer was inserted after the selector plug. Effects of plug-plug filling on enantiomers' migration and resolution are discussed. The ppPF-CE might be a new flexible mode for CE application.
在常见的部分填充毛细管电泳（PF-CE）中，毛细管在进样端和检测端之间含有选择性填充塞，以避免选择性试剂进入检测区域。为了扩展这种方法，我们提出了一种在毛细管内耦合两个不连续功能塞的模式，称为塞-塞PF-CE（ppPF-CE）。首先，我们提出了一种预定义手性选择剂有效长度以满足对映体分离要求的方法，这可以避免一些实验步骤。以α-环糊精作为手性选择剂，通过最佳长度和浓度的部分填充α-环糊精塞，获得了对映体d，l-色氨酸和d，l-酪氨酸的满意分离度。随后，在选择剂塞之后插入第二个塞，该塞包含羟丙基甲基纤维素、有机溶剂（乙腈和甲醇）、阴离子和阳离子表面活性剂（十二烷基硫酸钠和十六烷基三甲基溴化铵）以及不同浓度的磷酸钠缓冲液。讨论了塞-塞填充对对映体迁移和分离度的影响。ppPF-CE可能是毛细管电泳应用中的一种新的灵活模式。
