Tesarová Eva, Sevcík Juraj, Gas Bohuslav, Armstrong Daniel W
Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Prague, Czech Republic.
Electrophoresis. 2004 Aug;25(16):2693-700. doi: 10.1002/elps.200405972.
The effect of several experimental parameters on enantiomeric separations in micellar capillary electrophoresis (MCE) was studied. A model separation system was tested. It was composed of an acidic phosphate buffer with sodium dodecyl sulfate (SDS) and hydroxypropyl-beta-cyclodextrin (HP-beta-CD) as the chiral selector. A substituted angelicin was used as a chiral analyte. Changes in the concentration of SDS micelles/SDS monomers in the presence of HP-beta-CD and their impact on the enantioselective separation were investigated. Variation of the composition of electrolytes in the individual compartments of the separation system (inlet vial, capillary, and outlet vial) affected both the migration times and the resolution of the enantiomers. Current vs. time dependencies also were monitored during the separations. A mathematical model of electromigration in micellar systems with chiral selector present was proposed and a computer simulation was used to explain the observed phenomena and to confirm the generation of a CD/SDS-micelle concentration gradient under certain experimental conditions. This is the reported first attempt of a computer simulation of the complex, dynamic chiral environment of the CD-SDS-MCE system.
研究了几个实验参数对胶束毛细管电泳（MCE）中对映体分离的影响。测试了一个模型分离系统。它由一种酸性磷酸盐缓冲液组成，其中含有十二烷基硫酸钠（SDS）和羟丙基-β-环糊精（HP-β-CD）作为手性选择剂。一种取代白芷素用作手性分析物。研究了在HP-β-CD存在下SDS胶束/SDS单体浓度的变化及其对对映体选择性分离的影响。分离系统各个隔室（进样瓶、毛细管和出样瓶）中电解质组成的变化影响了对映体的迁移时间和分离度。在分离过程中还监测了电流与时间的相关性。提出了在手性选择剂存在的胶束系统中电迁移的数学模型，并通过计算机模拟来解释观察到的现象，并证实了在某些实验条件下会产生CD/SDS-胶束浓度梯度。这是首次报道对CD-SDS-MCE系统复杂、动态手性环境进行计算机模拟的尝试。
