Li Chengjun, Lu Yaoyao, Ma Shangshang, Lü Peng, Li Bin, Chen Keping
Institute of Life Sciences, Jiangsu University, Zhenjiang, China.
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, China.
Arch Insect Biochem Physiol. 2018 Oct;99(2):e21496. doi: 10.1002/arch.21496. Epub 2018 Jul 9.
Crinkled is associated with embryonic denticle formation and auditory organ development in Drosophila melanogaster. However, the functions of Crinkled have not been fully investigated. Additionally, the genes that participate in the Crinkled pathway are unknown. Phylogenetic analysis indicates that crinkled exhibits a one-to-one orthologous relationship in insects. In Tribolium castaneum, the crinkled gene is 6,498 bp in length and consists of six exons. Crinkled expression peaked during two phases in Tribolium: late embryonic and pupal stages. High levels of crinkled mRNA were detected in the fat body, head, epidermis, ovary, and accessory gland of late adults. Knockdown of crinkled using RNA interference (RNAi) severely affected wing morphogenesis in T. castaneum. We further showed that crinkled silencing reduced forked expression through wingless and shaven-baby, and RNAi of forked phenocopied the effects of crinkled knockdown in T. castaneum. This study investigated the development role of crinkled in postembryonic stages and indicated that forked mediates the functions of crinkled during wing morphogenesis in T. castaneum.
“皱缩”与黑腹果蝇的胚胎齿形成及听觉器官发育有关。然而，“皱缩”的功能尚未得到充分研究。此外，参与“皱缩”途径的基因也未知。系统发育分析表明，“皱缩”在昆虫中呈现一对一的直系同源关系。在赤拟谷盗中，“皱缩”基因长度为6498 bp，由六个外显子组成。“皱缩”的表达在赤拟谷盗的两个阶段达到峰值：胚胎后期和蛹期。在成虫后期的脂肪体、头部、表皮、卵巢和附腺中检测到高水平的“皱缩”mRNA。使用RNA干扰（RNAi）敲低“皱缩”会严重影响赤拟谷盗的翅形态发生。我们进一步表明，“皱缩”沉默通过无翅和光裸宝贝降低了叉状表达，并且叉状RNAi模拟了赤拟谷盗中“皱缩”敲低的效果。本研究调查了“皱缩”在胚胎后期发育中的作用，并表明叉状在赤拟谷盗翅形态发生过程中介导“皱缩”的功能。
