State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, College of Biotechnology, Southwest University, Chongqing, China.
Insect Sci. 2022 Feb;29(1):65-77. doi: 10.1111/1744-7917.12911. Epub 2021 Apr 6.
The development of insect appendages requires the expression of multiple genes in a strict spatial and temporal order. The odd-skipped family genes are vital transcriptional factors involved in embryonic development. The development and morphogenesis of the insect wing requires multiple transcription factors to regulate the expression of wing patterning genes at the transcriptional level. However, the function of odd-related genes in insect wing morphogenesis and development during postembryonic stages is unclear. We focused on the roles of the sister of odd and bowl (sob) gene, a member of odd-skipped family genes, during the wing morphopoiesis in Bombyx mori using the clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 system and in Tribolium castaneum by RNA interference. The results showed that the wings were significantly smaller and degenerated, and wing veins were indistinct in the sob gene loss-of-function group in both B. mori and T. castaneum. Quantitative real-time polymerase chain reaction revealed that the Tcsob gene regulated the expression of wing development genes, such as the cht 7 and the vg gene. The findings suggest the importance of sob gene in insect wing morphology formation during postembryonic stages.
昆虫附肢的发育需要多个基因在严格的时空顺序中表达。奇数跳跃家族基因是参与胚胎发育的重要转录因子。昆虫翅膀的发育和形态发生需要多个转录因子在转录水平上调节翅膀模式基因的表达。然而，odd-related 基因在昆虫胚胎后阶段翅膀形态发生和发育中的功能尚不清楚。我们使用簇状规律间隔的短回文重复序列（CRISPR）/CRISPR 相关蛋白 9 系统聚焦于 odd-skipped 家族基因的姐妹基因 odd 和 bowl（sob）在鳞翅目昆虫家蚕（Bombyx mori）和鞘翅目昆虫赤拟谷盗（Tribolium castaneum）翅膀形态发生中的作用，通过 RNA 干扰。结果表明，在 B. mori 和 T. castaneum 的 sob 基因功能丧失组中，翅膀明显变小、退化，翅膀脉纹不明显。实时定量聚合酶链反应显示，Tcsob 基因调节了 cht 7 和 vg 等翅膀发育基因的表达。这些发现表明 sob 基因在昆虫胚胎后阶段翅膀形态形成中的重要性。
