Huan Jun, Dou Lei, Yan Qi-Fang, Yang De-Qin
Dept. of Conservative Dentistry and Endodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China;Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China;Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2018 Jun 1;36(3):247-251. doi: 10.7518/hxkq.2018.03.003.
This study aimed to explore the effects of concentrate growth factor extracts (CGFe) on human umbilical vein endothelial cells (HUVECs) in vitro.
Concentrate growth factor (CGF) were prepared from the peripheral blood of healthy donors, followed by CGFe. Four groups were designed based on cell culture medium, as follows: 2%CGFe, 5%CGFe, 10%CGFe, and control. The proliferation activity of HUVECs was detected by cell cycle and CCK-8 assays. The migration of HUVECs was detected by scratch assay. The mRNA expression levels of vascular endothelial growth factor (VEGF), chemokine receptor 4 (CXCR4), and platelet derived growth factor (PDGF) were examined by quantitative real time polymerase chain reaction (qRT-PCR).
RESULTS: Results of CCK-8 and cell cycle assays showed that CGFe promoted the proliferation capability of HUVECs in a dose-dependent manner, and the data had statistical significance among four groups (P< 0.05). The cell migration assay indicated that CGF accelerated wound closure in a dose-dependent manner after 12 h of culture (P<0.05). The results of qRT-PCR showed that CGF upregulated the expression levels of VEGF, CXCR4, and PDGF in HUVECs.
CGFe can promote the proliferation, migration, and angiogenic differentiation of HUVECs. Thus, CGF might be an appropriate cure for dental pulp revascularization.
本研究旨在探讨浓缩生长因子提取物（CGFe）对人脐静脉内皮细胞（HUVECs）的体外作用。
从健康供体的外周血中制备浓缩生长因子（CGF），进而得到CGFe。根据细胞培养基设计四组，如下：2%CGFe、5%CGFe、10%CGFe和对照组。通过细胞周期和CCK-8检测法检测HUVECs的增殖活性。通过划痕试验检测HUVECs的迁移情况。通过定量实时聚合酶链反应（qRT-PCR）检测血管内皮生长因子（VEGF）、趋化因子受体4（CXCR4）和血小板衍生生长因子（PDGF）的mRNA表达水平。
CCK-8和细胞周期检测结果显示，CGFe以剂量依赖的方式促进HUVECs的增殖能力，四组数据具有统计学意义（P<0.05）。细胞迁移试验表明，培养12小时后，CGF以剂量依赖的方式加速伤口愈合（P<0.05）。qRT-PCR结果显示，CGF上调HUVECs中VEGF、CXCR4和PDGF的表达水平。
CGFe可促进HUVECs的增殖、迁移和血管生成分化。因此，CGF可能是牙髓血管再生的一种合适治疗方法。
