Honda Hirotsugu, Tamai Noriyuki, Naka Norifumi, Yoshikawa Hideki, Myoui Akira
Medical Center for Translational and Clinical Research, Department of Medical Innovation, Osaka University Hospital, 2-15 Yamadaoka, Suita, Osaka, 565-0871, Japan,
J Artif Organs. 2013 Sep;16(3):305-15. doi: 10.1007/s10047-013-0711-7. Epub 2013 May 23.
Concentrated growth factor (CGF) is an autologous leukocyte-rich and platelet-rich fibrin (L-PRF) biomaterial termed "second-generation platelet concentrate". CGF contains autologous osteoinductive platelet growth factors and an osteoconductive fibrin matrix. The purpose of this study was to assess the ability of CGF combined with bone marrow stromal cells (BMSCs) to heal critical-size rat calvaria defects in vivo and to modulate the proliferation and osteogenic differentiation of mesenchymal stem cells (MSCs) in vitro. In the in-vivo study, the CGF group regenerated bone better than the control group, and combined therapy with CGF and BMSCs almost completely repaired critical-size bone defects within 12 weeks after surgery. In the in-vitro study, the CGF extract, at concentrations between 1 and 10%, promoted proliferation, osteogenic maturation, and mineralization of hTERT-E6/E7 human MSCs in a dose-dependent manner but had an inhibitory effect at higher concentrations. In conclusion, a CGF extract promoted the proliferation, osteogenic maturation, and mineralization of mesenchymal stem cells in vitro, and combination therapy with CGF and BMSCs resulted in excellent healing of critical-size bone defects in vivo.
浓缩生长因子（CGF）是一种自体富含白细胞和富含血小板的纤维蛋白（L-PRF）生物材料，被称为“第二代血小板浓缩物”。CGF含有自体骨诱导性血小板生长因子和骨传导性纤维蛋白基质。本研究的目的是评估CGF联合骨髓基质细胞（BMSCs）在体内修复大鼠颅骨临界尺寸缺损的能力，以及在体外调节间充质干细胞（MSCs）增殖和成骨分化的能力。在体内研究中，CGF组的骨再生情况优于对照组，CGF与BMSCs联合治疗在术后12周内几乎完全修复了临界尺寸的骨缺损。在体外研究中，浓度为1%至10%的CGF提取物以剂量依赖的方式促进了hTERT-E6/E7人MSCs的增殖、成骨成熟和矿化，但在较高浓度时具有抑制作用。总之，CGF提取物在体外促进了间充质干细胞的增殖、成骨成熟和矿化，CGF与BMSCs联合治疗在体内导致临界尺寸骨缺损的良好愈合。
