Herrmann A, Arnold K, Pratsch L
Biosci Rep. 1985 Aug;5(8):689-96. doi: 10.1007/BF01117001.
A drastic increase of the intracellular microviscosity of red blood cells in the presence of polyethylene glycol (PEG) was established by electron spin resonance using the small spin label molecule 2,2,6,6-tetramethyl-piperidine-N-oxyl-4-one (TEMPONE). The effective osmotic pressure of PEG solutions stressing the cells was estimated by comparison with those cytoplasmic rotational correlation times of TEMPONE measured in NaCl or sucrose containing media of known osmotic pressure.
通过电子自旋共振，利用小自旋标记分子2,2,6,6-四甲基哌啶-N-氧基-4-酮（TEMPONE），证实了在聚乙二醇（PEG）存在下红细胞细胞内微粘度急剧增加。通过与在已知渗透压的含NaCl或蔗糖介质中测量的TEMPONE的细胞质旋转相关时间进行比较，估算了使细胞产生应激的PEG溶液的有效渗透压。
