Dikalov S, Skatchkov M, Bassenge E
Institute of Chemical Kinetics & Combustion, Novosibirsk, Russia.
Biochem Biophys Res Commun. 1997 Jan 3;230(1):54-7. doi: 10.1006/bbrc.1996.5880.
The reactions of hydroxylamine 1-hydroxy-2,2,6,6-tetramethyl-4-oxo-piperidine hydrochloride (TEMPONE-H) with peroxynitrite, superoxide and peroxyl radicals were studied. It was shown that under these reactions TEMPONE-H is oxidized into a stable nitroxide 1-hydroxy-2,2,6,6-tetramethyl-4-oxo-piperidi-noxyl (TEMPONE). The reactivity of TEMPONE-H towards reactive oxygen species was compared with the spin traps DMPO and TMIO as well as with DMSO and SOD. The rate constants of reactions of TEMPONE-H with peroxynitrite and superoxide radicals were 6 x 10(9) M(-1)s(-1) and 1.2x10(4) M(-1)s(-1), respectively. Using TEMPONE-H the sensitivity in the detection of peroxynitrite or superoxide radical was about 10-fold higher than using the spin traps DMPO or TMIO. Thus, TEMPONE-H may be used as a spin trap in chemical and biological systems to quantify peroxynitrite and superoxide radical formation.
研究了盐酸羟胺1-羟基-2,2,6,6-四甲基-4-氧代哌啶（TEMPONE-H）与过氧亚硝酸盐、超氧阴离子和过氧自由基的反应。结果表明，在这些反应中，TEMPONE-H被氧化为稳定的氮氧自由基1-羟基-2,2,6,6-四甲基-4-氧代哌啶氮氧自由基（TEMPONE）。将TEMPONE-H对活性氧的反应性与自旋捕获剂DMPO和TMIO以及二甲基亚砜（DMSO）和超氧化物歧化酶（SOD）进行了比较。TEMPONE-H与过氧亚硝酸盐和超氧阴离子自由基反应的速率常数分别为6×10⁹ M⁻¹s⁻¹和1.2×10⁴ M⁻¹s⁻¹。使用TEMPONE-H检测过氧亚硝酸盐或超氧阴离子自由基的灵敏度比使用自旋捕获剂DMPO或TMIO高约10倍。因此，TEMPONE-H可作为化学和生物系统中的自旋捕获剂，用于定量过氧亚硝酸盐和超氧阴离子自由基的形成。
