INFIQC (CONICET), Haya de la Torre s/n, Ciudad Universitaria , X5000HUA Córdoba , Argentina.
Departamento de Fisicoquímica, Facultad de Ciencias Químicas , Universidad Nacional de Córdoba, Ciudad Universitaria , X5000HUA Córdoba , Argentina.
ACS Appl Mater Interfaces. 2018 Jul 18;10(28):23501-23508. doi: 10.1021/acsami.8b03039. Epub 2018 Jul 9.
We report the first optical biosensor for the novel and important cardiac biomarker, galectin-3 (Gal3), using the anti-Gal3 antibody as a biorecognition element and surface plasmon resonance (SPR) for transducing the bioaffinity event. The immunosensing platform was built at a thiolated Au surface modified by self-assembling four bilayers of poly(diallyldimethylammonium chloride) and graphene oxide (GO), followed by the covalent attachment of 3-aminephenylboronic acid (3ABA). The importance of GO, both as the anchoring point of the antibody and as a field enhancer for improving the biosensor sensitivity, was critically discussed. The advantages of using 3ABA to orientate the anti-Gal3 antibody through the selective link to the Fc region were also demonstrated. The new platform represents an interesting alternative for the label-free biosensing of Gal3 in the whole range of clinically relevant concentrations (linear range between 10.0 and 50.0 ng mL, detection limit of 2.0 ng mL) with successful application for Gal3 biosensing in enriched human serum samples.
我们报告了第一个光学生物传感器，用于新型重要的心脏生物标志物半乳糖凝集素-3（Gal3），使用抗 Gal3 抗体作为生物识别元件，表面等离子体共振（SPR）用于转换生物亲和事件。免疫传感平台构建在经过巯基化的 Au 表面上，该表面通过自组装聚（二烯丙基二甲基氯化铵）和氧化石墨烯（GO）的四层修饰，然后通过共价键连接 3-氨苯基硼酸（3ABA）。还重点讨论了 GO 的重要性，它既是抗体的附着点，也是增强场以提高生物传感器灵敏度的增强剂。还证明了使用 3ABA 通过与 Fc 区域的选择性连接来定向抗 Gal3 抗体的优势。该新平台代表了在整个临床相关浓度范围内（线性范围在 10.0 和 50.0 ng mL 之间，检测限为 2.0 ng mL）无标记 Gal3 生物传感的一种有趣的替代方法，并成功应用于富含人血清样本中的 Gal3 生物传感。
