INFIQC-CONICET, Departamento de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000, Córdoba, Argentina.
Chimie ParisTech CNRS 2027, Institute of Chemistry for Life and Health Sciences SEISAD, PSL University, 75005, Paris, France.
Anal Bioanal Chem. 2020 Jun;412(15):3539-3546. doi: 10.1007/s00216-020-02593-w. Epub 2020 Apr 1.
This work is focused on the development of a genosensor for microRNA-21 quantification using surface plasmon resonance (SPR) to transduce the hybridization event. The biosensing platform was built by self-assembling two bilayers of poly(diallyldimethylammonium chloride) (PDDA) and graphene oxide (GO) at a gold surface modified with 3-mercaptopropane sulfonate (MPS), followed by the covalent attachment of the DNA probe. GO was used in two directions, to allow the anchoring of the probe DNA and to increase the sensitivity of the biosensing event due to its field enhancer effect. The new bioanalytical platform represents an interesting alternative for the label-free biosensing of microRNA-21, with a linear range between 1.0 fM and 10 nM, a sensitivity of 5.1 ± 0.1 mM and a detection limit of 0.3fM. The proposed sensing strategy was successfully used for the quantification of microRNA-21 in enriched urine samples. Graphical abstract.
这项工作专注于开发一种基于表面等离子体共振（SPR）的用于 microRNA-21 定量的基因传感器，以转导杂交事件。生物传感平台是通过自组装两层聚二烯丙基二甲基氯化铵（PDDA）和氧化石墨烯（GO）在巯基丙磺酸（MPS）修饰的金表面上构建的，然后通过 DNA 探针的共价连接。GO 被用于两个方向，以允许探针 DNA 的固定，并由于其场增强效应而提高生物传感事件的灵敏度。新的生物分析平台代表了一种用于 microRNA-21 的无标记生物传感的有趣替代方案，其线性范围在 1.0 fM 和 10 nM 之间，灵敏度为 5.1 ± 0.1 mM，检测限为 0.3 fM。所提出的传感策略成功地用于富集尿液样本中 microRNA-21 的定量。
