G6H1 事件转基因水稻事件特异性实时荧光定量 PCR 方法的建立及实验室间验证
Development and Interlaboratories Validation of Event-Specific Quantitative Real-Time PCR Method for Genetically Modified Rice G6H1 Event.
机构信息
National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology , Shanghai Jiao Tong University , Shanghai 200240 , China.
Biotechnology Research Institute , Chinese Academy of Agricultural Sciences , Beijing 100081 , China.
出版信息
J Agric Food Chem. 2018 Aug 1;66(30):8179-8186. doi: 10.1021/acs.jafc.8b01519. Epub 2018 Jul 18.
The transgenic rice G6H1 was a new event with the traits of herbicide-tolerance and insect-resistant. Herein, we developed one event-specific real-time PCR method with high specificity and sensitivity for G6H1 event quantitative analysis, and validated its performance on practical samples quantification through a collaborative ring trial. A total of eight laboratories participated in this validation and quantified three blind G6H1 powder samples including DNA extraction and real-time PCR analysis. The statistically analyzed results from returned data confirmed its high PCR efficiency and good linearity, trueness, and precision, indicating that the developed G6H1 real-time PCR assay was accurate, reliable, and comparable for G6H1 identification and quantification.
转基因为抗虫和耐除草剂的 G6H1 水稻是一个新的事件。在此，我们开发了一种具有高特异性和灵敏度的 G6H1 事件实时 PCR 方法，用于 G6H1 事件的定量分析，并通过合作环试验验证了其在实际样品定量分析中的性能。共有 8 个实验室参与了此次验证，并对包括 DNA 提取和实时 PCR 分析在内的 3 个 G6H1 粉末盲样进行了定量分析。从返回数据进行的统计分析结果证实了其高 PCR 效率和良好的线性、准确性和精密度，表明开发的 G6H1 实时 PCR 检测方法准确、可靠、可用于 G6H1 的鉴定和定量。
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