Yunnan Institute of Food Safety, Kunming University of Science and Technology, Yunnan 650500, China.
Beijing Laboratory of Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Molecules. 2019 Jan 28;24(3):455. doi: 10.3390/molecules24030455.
is a rare, precious, and wild edible fungus that could not be cultivated artificially until now. This situation has given way to the introduction of fake commodities to the mushroom market. Among the methods used to detect food adulteration, amplification of species-specific diagnostic marker is particularly important and accurate. In this study, the gene is reported as a species-specific diagnostic marker to identify three varieties and 10 other types of edible mushrooms through qualitative and quantitative PCR. The PCR results did not reveal variations in the amplified region, and the detection limits of qualitative and quantitative PCR were found to be 8 ng and 32 pg, respectively. Southern blot showed that the Pol gene exists as a single copy in the genome. The method that produced the purest DNA of in this study was also determined, and the high-concentration salt precipitation method was confirmed to be the most suitable among the methods tested. The assay proposed in this work is applicable not only to the detection of raw materials but also to the examination of processed products containing .
是一种珍稀、珍贵且野生的食用真菌，至今仍无法进行人工栽培。这种情况导致了假冒商品进入蘑菇市场。在用于检测食品掺假的方法中，扩增物种特异性诊断标记物尤为重要且准确。在本研究中，该基因被报道为一种物种特异性诊断标记物，可通过定性和定量 PCR 来鉴定三种品种和其他十种食用蘑菇。PCR 结果未显示扩增区域的变化，定性和定量 PCR 的检测限分别为 8ng 和 32pg。Southern blot 显示 Pol 基因在基因组中以单拷贝形式存在。本研究还确定了获得最纯 的 DNA 的方法，高浓度盐沉淀法被证实是所有测试方法中最适合的方法。本工作提出的测定方法不仅适用于原材料的检测，也适用于含 的加工产品的检查。
