Giancotti V, Berlingieri M T, DiFiore P P, Fusco A, Vecchio G, Crane-Robinson C
Cancer Res. 1985 Dec;45(12 Pt 1):6051-7.
Two-dimensional electrophoresis has been used to document changes in nuclear proteins following viral transformation of an epithelial cell line exhibiting differentiation markers. After transformation, these markers are lost, and the cells become tumorigenic and capable of growth in soft agar. A sharp rise in the phosphorylation of histones H1, H2A, and ubiquitinated H2A is seen on transformation, together with the appearance of three phosphorylated proteins that are extractable by perchloric acid and appear related to high mobility group Protein 14, a constituent of active chromatin. Since comparison is made between normal and transformed cells that are each grown to confluence and since there is little difference between their observed growth rates, the changes seen represent intrinsic differences between the cell lines and are thus a direct reflection of the process of transformation.
二维电泳已被用于记录一种表现出分化标志物的上皮细胞系经病毒转化后核蛋白的变化。转化后，这些标志物消失，细胞变成致瘤性的且能够在软琼脂中生长。转化时可见组蛋白H1、H2A以及泛素化H2A的磷酸化急剧增加，同时出现三种可被高氯酸提取的磷酸化蛋白，它们似乎与活性染色质的组成成分高迁移率族蛋白14有关。由于是在均生长至汇合状态的正常细胞和转化细胞之间进行比较，且它们观察到的生长速率几乎没有差异，所以观察到的变化代表了细胞系之间的内在差异，因此是转化过程的直接反映。
