重症肌无力中神经毒素结合抑制性抗体的动力学研究。
Kinetic studies on neurotoxin-binding inhibitory antibodies in myasthenia gravis.
作者信息
Ito A, Furukawa S, Satoyoshi E, Sakurai H
出版信息
Clin Chim Acta. 1985 Nov 15;152(3):315-23. doi: 10.1016/0009-8981(85)90107-x.
A method for measuring antibodies which inhibit the binding of 125I-alpha-bungarotoxin to the acetylcholine receptor was developed. The procedure allows the detection of inhibitory activity in 70% of sera from the patients with myasthenia gravis. Kinetic analysis showed that (1) a linear dose-response exists between logarithmic concentrations of any test serum and inhibitory activity, (2) the slope of the inhibition line is identical among various myasthenic sera and, (3) the slope of line induced by various cholinergic ligands was comparable to that obtained by myasthenic sera. The inhibitory activity correlated with the titer of anti-acetylcholine receptor antibody measured by an immunoprecipitation method (r = 0.76, p less than 0.01).
开发了一种测量抑制¹²⁵I - α - 银环蛇毒素与乙酰胆碱受体结合的抗体的方法。该程序可检测70%重症肌无力患者血清中的抑制活性。动力学分析表明：（1）任何测试血清的对数浓度与抑制活性之间存在线性剂量反应；（2）不同重症肌无力血清的抑制线斜率相同；（3）各种胆碱能配体诱导的线斜率与重症肌无力血清获得的斜率相当。抑制活性与通过免疫沉淀法测得的抗乙酰胆碱受体抗体滴度相关（r = 0.76，p < 0.01）。
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