重症肌无力患者血清中抗乙酰胆碱受体抗体测定的放射免疫分析方法评估。

An assessment of radioimmunoassay procedures for determination of anti-acetylcholine receptor antibodies in the sera of patients with myasthenia gravis.

作者信息

Carter B, Harrison R, Lunt G G, Morris H, Savage-Marengo T, Stephenson F A

出版信息

Ann Clin Biochem. 1981 May;18(Pt 3):146-52. doi: 10.1177/000456328101800304.

Abstract

A reproducible radioimmunoassay procedure for the determination of anti-acetylcholine receptor antibodies in the sera of patients with myasthenia gravis is described and examined in detail. The assay combines features of a number of methods previously outlined and allows repeat determinations of antibody titre in a given myasthenic serum sample with coefficient of variation 6%. The mean +/- standard deviation for normal human serum anti-acetylcholine receptor antibodies was found by this procedure to be 0.024 +/- 0.033 nmol/l alpha-bungarotoxin binding sites whereas the range for myasthenic patients was 0-139.14 nmol/l with a mean value of 7.55 nmol/l alpha-bungarotoxin binding sites.

摘要

本文详细描述并检验了一种用于测定重症肌无力患者血清中抗乙酰胆碱受体抗体的可重复放射免疫分析方法。该分析方法结合了先前概述的多种方法的特点，能够对给定的重症肌无力血清样本中的抗体滴度进行重复测定，变异系数为6%。通过此方法发现，正常人血清抗乙酰胆碱受体抗体的平均值±标准差为0.024±0.033nmol/lα-银环蛇毒素结合位点，而重症肌无力患者的范围为0 - 139.14nmol/l，α-银环蛇毒素结合位点的平均值为7.55nmol/l。