Jellinck P H, Quail J A, Crowley C A
Endocrinology. 1985 Dec;117(6):2274-8. doi: 10.1210/endo-117-6-2274.
Human GH or recombinant DNA-derived human GH administered to normal mature male rats by constant infusion decreased hepatic 2-hydroxylation of estradiol to female levels, as measured by 3H2O release and isolation of the catechol estrogen product. PRL had no effect under the same conditions. The maximum response to GH was attained at an infusion rate of 0.02 IU/h . kg after 5-7 days, and a significant change was observed within 1-2 days. The effect of GH was primarily on hydroxylation of C-2 of the estrogen, as demonstrated by comparative studies with estradiol labeled with 3H at C-4 or C-6,7, and appeared to be mediated by the cytochrome P-450 system of the liver microsomes. Ascorbic acid at 1 mM did not affect 2-hydroxylation significantly while protecting the catechol estrogen produced from further oxidation. The results indicate that GH has the potential to regulate estrogen metabolism in the liver and provide evidence for another component in the hypothalamic-pituitary-liver axis.
通过持续输注向正常成熟雄性大鼠给药人生长激素（GH）或重组DNA衍生的人GH，可将雌二醇的肝脏2-羟化作用降低至雌性水平，这是通过3H2O释放和儿茶酚雌激素产物的分离来测定的。在相同条件下，催乳素（PRL）没有影响。在5-7天后，以0.02 IU/h·kg的输注速率可达到对GH的最大反应，并且在1-2天内观察到显著变化。与在C-4或C-6、7处用3H标记的雌二醇进行的比较研究表明，GH的作用主要是对雌激素C-2的羟化作用，并且似乎是由肝微粒体的细胞色素P-450系统介导的。1 mM的抗坏血酸在保护产生的儿茶酚雌激素不被进一步氧化的同时，对2-羟化作用没有显著影响。结果表明，GH有调节肝脏中雌激素代谢的潜力，并为下丘脑-垂体-肝脏轴中的另一个组成部分提供了证据。
