Allorent Guillaume, Guglielmino Erika, Giustini Cécile, Courtois Florence
Laboratoire de Physiologie Cellulaire et Végétale, Centre National de la Recherche Scientifique, Institut National Recherche Agronomique, Commissariat à l'Energie Atomique et aux Energies Alternatives, CEA Grenoble, UMR5168, Université Grenoble Alpes, Grenoble, France.
Methods Mol Biol. 2018;1829:367-378. doi: 10.1007/978-1-4939-8654-5_24.
Genome modifications in microalgae are becoming a widespread and mandatory tool for research in both fundamental and applied biology. Among genome editing methods in these photosynthetic organisms, CRISPR/Cas9 offers a specific, powerful and efficient tool for genome engineering by inducing mutations in targeted regions of the genome. Here we described a protocol that allows the generation of knockout mutants by CRISPR/Cas9 in the diatom Phaeodactylum tricornutum using biolistic transformation.
微藻中的基因组修饰正成为基础生物学和应用生物学研究中一种广泛且必不可少的工具。在这些光合生物的基因组编辑方法中，CRISPR/Cas9通过在基因组的靶向区域诱导突变，为基因组工程提供了一种特异、强大且高效的工具。在此，我们描述了一种方案，该方案允许使用基因枪转化法，通过CRISPR/Cas9在硅藻三角褐指藻中产生基因敲除突变体。
