Stober Vandy P, Garantziotis Stavros
Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA.
Methods Mol Biol. 2018;1809:301-314. doi: 10.1007/978-1-4939-8570-8_19.
Ozone is a major pollutant in the air we breathe, and elevated levels lead to significant morbidity and mortality. As the climate warms, levels of ozone are predicted to increase. Accordingly, studies to assess the mechanisms of ozone-induced lung diseases are paramount. This chapter describes mouse models of ozone exposure and methods for assessing the effects of ozone in the lungs. These include bronchoalveolar lavage, necropsy, and measurement of lung function. Lavage allows for assessment of cell infiltration, cytokine production, tissue damage and capillary leakage in the airspaces. Necropsy provides tissue for gene expression, histology, and protein assessment in the whole lung. Lung physiology is used to assess airway hyperresponsiveness, tissue and total lung resistance, compliance, and elastance.
臭氧是我们呼吸的空气中的一种主要污染物,其浓度升高会导致显著的发病率和死亡率。随着气候变暖,预计臭氧水平将会上升。因此,评估臭氧诱发肺部疾病机制的研究至关重要。本章描述了臭氧暴露的小鼠模型以及评估臭氧对肺部影响的方法。这些方法包括支气管肺泡灌洗、尸检以及肺功能测量。灌洗可用于评估气腔内的细胞浸润、细胞因子产生、组织损伤和毛细血管渗漏。尸检可为全肺的基因表达、组织学和蛋白质评估提供组织样本。肺生理学用于评估气道高反应性、组织和全肺阻力、顺应性和弹性。